The enzyme D-serine ammonia-lyase (EC 4.3.1.18), with systematic name D-serine ammonia-lyase (pyruvate-forming), catalyzes the chemical reaction

D-serine = pyruvate + NH₃ (overall reaction)

(1a) D-serine = 2-aminoprop-2-enoate + H₂O

(1b) 2-aminoprop-2-enoate = 2-iminopropanoate (spontaneous)

(1c) 2-iminopropanoate + H₂O = pyruvate + NH₃ (spontaneous

Other names in common use include D-hydroxyaminoacid dehydratase, D-serine dehydrase, D-hydroxy amino acid dehydratase, D-serine hydrolase, D-serine dehydratase (deaminating), D-serine deaminase, and D-serine hydro-lyase (deaminating). This enzyme participates in glycine, serine and threonine metabolism. It employs one cofactor, pyridoxal phosphate.

• Dupourque D, Newton WA, Snell EE (1966). "Purification and properties of D-serine dehydrase from Escherichia coli". J. Biol. Chem. 241 (5): 1233–8. doi:10.1016/S0021-9258(18)96825-0. PMID 5327100.
• METZLER DE, SNELL EE (1952). "Deamination of serine. II. D-Serine dehydrase, a vitamin B₆ enzyme from Escherichia coli". J. Biol. Chem. 198 (1): 363–73. doi:10.1016/S0021-9258(18)55590-3. PMID 12999751.