Competition-ChIP

Competition-ChIP is variant of the Chip-Sequencing protocol, used to measure relative binding dynamics of a transcription factor (TF) on DNA.[1] Since TF occupancy measures are thought to be a poor predictor of TF function at a given locus, Competition-ChIP is much more strongly linked to function than occupancy. The technique was originally developed in Jason D. Lieb's Lab in 2011.

References

  1. ^ Lieb, Jason D. (2013). "Genome-wide measurement of protein-DNA binding dynamics using competition ChIP". Nat. Protoc. 8 (7): 1337–53. doi:10.1038/nprot.2013.077. PMID 23764940. S2CID 28799014.

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