Apo-beta-carotenoid-14',13'-dioxygenase (EC 1.13.11.67 is an enzyme that catalyzes the chemical reaction

8'-apo-beta-carotenol + O₂ ${\displaystyle \rightleftharpoons }$ 14'-apo-beta-carotenal + uncharacterized product

Thus, the two substrates of this enzyme are 8'-apo-beta-carotenol and oxygen, whereas its two products are 14'-apo-beta-carotenal and an uncharacterized product that may be (3E,5E)-7-hydroxy-6-methylhepta-3,5-dien-2-one.

This enzyme belongs to the family of oxidoreductases, specifically those acting on single donors with O₂ as oxidant and incorporation of two atoms of oxygen into the substrate (oxygenases). The oxygen incorporated need not be derived from O with incorporation of one atom of oxygen (internal monooxygenases o internal mixed-function oxidases). The systematic name of this enzyme class is 8'-apo-beta-carotenol:O2 oxidoreductase.

• Daruwalla, A.; Zhang, J.; Lee, H. J.; Khadka, N.; Farquhar, E. R.; Shi, W.; von Lintig, J.; Kiser, P. D. (2020). "Structural basis for carotenoid cleavage by an archaeal carotenoid dioxygenase". Proceedings of the National Academy of Sciences of the United States of America. 117 (33): 19914–19925. Bibcode:2020PNAS..11719914D. doi:10.1073/pnas.2004116117. PMC 7443882. PMID 32747548.
• Dmitrovskii AA, Gessler NN, Gomboeva SB (July 1997). "Enzymatic oxidation of beta-apo-8'-carotenol to beta-apo-14'-carotenal by an enzyme different from beta-carotene-15,15'-dioxygenase". Biochemistry. Biokhimiia. 62 (7): 787–92. PMID 9331970.