In enzymology, an alkylglycerol kinase (EC 2.7.1.93) is an enzyme that catalyzes the chemical reaction

ATP + 1-O-alkyl-sn-glycerol ${\displaystyle \rightleftharpoons }$ ADP + 1-O-alkyl-sn-glycerol 3-phosphate

Thus, the two substrates of this enzyme are ATP and 1-O-alkyl-sn-glycerol, whereas its two products are ADP and 1-O-alkyl-sn-glycerol 3-phosphate.

This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. The systematic name of this enzyme class is ATP:1-O-alkyl-sn-glycerol 3-phosphotransferase. Other names in common use include 1-alkylglycerol kinase (phosphorylating), ATP-alkylglycerol phosphotransferase, alkylglycerol phosphotransferase, and ATP: 1-alkyl-sn-glycerol phosphotransferase. This enzyme participates in ether lipid metabolism.

• Rock CO, Snyder F (1974). "Biosynthesis of 1-alkyl-sn-glycero-3-phosphate via adenosine triphosphate:1-alkyl-sn-glycerol phosphotransferase". J. Biol. Chem. 249 (17): 5382–7. PMID 4369816.