Prudenti and her future husband, Robert Cimino, clerked for Suffolk surrogate judge Ernest L. Signorelli.[2] She later worked for the Suffolk County assistant district attorney for two years.[5] She worked in private practice for ten years.[5] Prudenti served as special counsel for the New York Police and Fire Widows' and Children's Benefit Fund.[5] In 1991, she was elected to the New York Supreme Court.[5] Prudenti served until 1995.[5] Prudenti was the Suffolk surrogate judge.[5] She was the first female elected to this role.[5] In 1996, she concurrently served as an acting New York Supreme Court justice.[5] In February 1999, she was appointed by chief administrative judge Jonathan Lippman to become the first person to dually serve as the Tenth Judicial District, Suffolk County administrative judge.[3][5] Prudenti was the first New York surrogate to serve as a district administrative judge.[5]
On September 1, 2015, Prudenti joined the Maurice A. Deane School of Law as the executive director of its center for children, families, and the law and senior associate dean of operations.[8][5] On January 1, 2017, she became the interim dean, succeeding Eric Lane.[8][10] She became the 10th dean in May 2017.[8][4] In January 2023, Prudenti announced she would step down on June 30.[4]