In enzymology, a 6-carboxyhexanoate—CoA ligase (EC 6.2.1.14) is an enzyme that catalyzes the chemical reaction

ATP + 6-carboxyhexanoate + CoA ${\displaystyle \rightleftharpoons }$ AMP + diphosphate + 6-carboxyhexanoyl-CoA

The 3 substrates of this enzyme are ATP, 6-carboxyhexanoate, and CoA, whereas its 3 products are AMP, diphosphate, and 6-carboxyhexanoyl-CoA.

This enzyme belongs to the family of ligases, specifically those forming carbon-sulfur bonds as acid-thiol ligases. The systematic name of this enzyme class is 6-carboxyhexanoate:CoA ligase (AMP-forming). Other names in common use include 6-carboxyhexanoyl-CoA synthetase, and pimelyl-CoA synthetase. This enzyme participates in biotin metabolism.

• Izumi Y, Morita H, Sato K, Tani Y, Ogata K (1972). "Synthesis of biotin-vitamers from pimelic acid and coenzyme A by cell-free extracts of various bacteria". Biochim. Biophys. Acta. 264 (1): 210–3. doi:10.1016/0304-4165(72)90133-x. PMID 4623286.
• Izumi Y, Morita H, Tani Y, Ogata K (1974). "The pimelyl-CoA synthetase responsible for the first step in biotin biosynthesis by microorganisms". Agric. Biol. Chem. 38 (11): 2257–2262. doi:10.1271/bbb1961.38.2257.