The 33rd annual Japanese Regional Football League Competition took place from 21 November 2009 to 6 December 2009. It took place across the prefectures of Fukushima, Toyama, Tottori, Kōchi and Nagano. It is the tournament which decided promotion to the Japan Football League for the 2010 season. The top two teams in this competition (Matsumoto Yamaga and Hitachi Tochigi Soccer Club) were given promotion to the Japan Football League. Third place Zweigen Kanazawa achieved promotion via a two-leg play off with F.C. Kariya.

Preliminary round - Four groups of four teams play each other once in a round-robin tournament. The top placed team in each group advances to the final round. Final Round - The four winners from the preliminary round play each other once in a round-robin tournament.

Three points are awarded for a win in standard time and zero for a lose. If at the end of standard time the result is a tie, a penalty shoot-out is held; the winning team is awarded 2 points and the losing team 1.

If the number of points are the same, the league position is ordered by goal difference, then the number of goals scored, and finally the result between the respective teams. If the 1st-place position cannot be decided by these factors, a play off will be contested between top two teams.

Preliminary round

• Group A - Iwaki Greenfield, Iwaki, Fukushima prefecture
• Group B - Takaoka Sports Core, Takaoka, Toyama prefecture
• Group C - Tottori Athletics Stadium, Tottori, Tottori prefecture
• Group D - Kochi Haruno Athletic Stadium, Kōchi, Kōchi Prefecture

Final round

• Matsumotodaira Football Stadium, Matsumoto, Nagano prefecture

• Hokkaido: Satsudai Goal Plunderers
• Tohoku: Grulla Morioka
• Kanto: Yokohama Sports and Culture Club
• Hokushinetsu: College of Upward Players in Soccer
• Tokai: Yazaki Valente
• Kansai: Sanyo Denki Sumoto Football Club
• Chugoku: Renofa Yamaguchi
• Shikoku: Tokushima Vortis Second
• Kyushu: Okinawa Kariyushi

Regional Leagues whose representative reached the final round in 2008, are eligible for a second team to represent them.

• Kanto: Hitachi Tochigi Soccer Club
• Chugoku: NTN Okayama S.C.
• Kyushu: Volca Kagoshima

• Matsumoto Yamaga (Hokushinetsu) (winner)
• Zweigen Kanazawa (Hokushinetsu) (runner-up)

• AS Laranja Kyoto (Kansai)
• Hamamatsu University (Tokai)

• Japan Football Association - The 33rd National Regional Football League Competition - Final Round (in Japanese)
• Japan Football Association - The 33rd National Regional Football League Competition - Schedule and Results (in Japanese)
• Japan Football Association - The 33rd National Regional Football League Competition - Tournament Outline (in Japanese)
• The Rec.Sport.Soccer Statistics Foundation - Japan Fourth and Lower Levels 2009