2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoate hydrolase

2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoate hydrolase (EC 3.7.1.13, CarC) is an enzyme with systematic name (2E,4E)-6-(2-aminophenyl)-2-hydroxy-6-oxohexa-2,4-dienoate acylhydrolase.^[1]^[2] This enzyme catalyses the following chemical reaction

(2E,4E)-6-(2-aminophenyl)-2-hydroxy-6-oxohexa-2,4-dienoate + H₂O

\rightleftharpoons

anthranilate + (2E)-2-hydroxypenta-2,4-dienoate

This enzyme catalyses the third step in the aerobic degradation pathway of carbazole.

References

^ Nojiri H, Taira H, Iwata K, Morii K, Nam JW, Yoshida T, Habe H, Nakamura S, Shimizu K, Yamane H, Omori T (January 2003). "Purification and characterization of meta-cleavage compound hydrolase from a carbazole degrader Pseudomonas resinovorans strain CA10". Bioscience, Biotechnology, and Biochemistry. 67 (1): 36–45. doi:10.1271/bbb.67.36. PMID 12619671.
^ Riddle RR, Gibbs PR, Willson RC, Benedik MJ (March 2003). "Purification and properties of 2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoic acid hydrolase involved in microbial degradation of carbazole". Protein Expression and Purification. 28 (1): 182–9. doi:10.1016/S1046-5928(02)00676-9. PMID 12651123.

External links

2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoate+hydrolase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

v t e Hydrolases: carbon-carbon (EC 3.7)
3.7.1	Fumarylacetoacetate hydrolase Kynureninase

v t e Enzymes
Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Diffusion-limited enzyme Cofactor Enzyme catalysis
Regulation	Allosteric regulation Cooperativity Enzyme inhibitor Enzyme activator
Classification	EC number Enzyme superfamily Enzyme family List of enzymes
Kinetics	Enzyme kinetics Eadie–Hofstee diagram Hanes–Woolf plot Lineweaver–Burk plot Michaelis–Menten kinetics
Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list) EC7 Translocases (list)

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