The enzyme 2-dehydropantoate aldolase (EC 4.1.2.12) catalyzes the chemical reaction:

2-dehydropantoate ${\displaystyle \rightleftharpoons }$ 3-methyl-2-oxobutanoate + formaldehyde

This enzyme belongs to the family of lyases, specifically the aldehyde-lyases, which cleave carbon-carbon bonds. The systematic name of this enzyme class is 2-dehydropantoate formaldehyde-lyase (3-methyl-2-oxobutanoate-forming). Other names in common use include ketopantoaldolase, and 2-dehydropantoate formaldehyde-lyase.

• McIntosh EN, Purko M, Wood WA (1957). "Ketopantoate formation by a hydroxymethylation enzyme from Escherichia coli". J. Biol. Chem. 228 (1): 499–510. PMID 13475336.