Tus

Tus protein z bakterie Escherichia coli navázaný na Ter místo na dvoušroubovici DNA[1]

Tus je monomerický protein účastnící se bakteriální replikace DNA. Gen tus kóduje bílkovinu, která se skládá z 308 aminokyselin (nepočítaje N-terminální methionin, jenž je odstraněn), molekulová hmotnost tus proteinu činí 35 652 daltonů.[2]

Je schopen ukončit (terminovat) replikaci. Váže se totiž na ukončovací (terminační) sekvence DNA, známé pod názvem Ter (TerA, TerB, TerC, TerD).[3] Tus však replikaci ukončuje pouze v jednom směru (v druhém směru jsou zase jiné tus proteiny) a dále vždy čeká, než replikační vidlice zcela dokončí replikaci (konkrétně dovolí vstup DnaB helikázy, ale nikoliv její další postup). Systém je však zřejmě poněkud komplikovanější.[2]

U některých jiných bakterií, jako je Bacillus subtilis, se vyskytují jiné zcela nepříbuzné proteiny, které však mají stejnou funkci. Konkrétně u B. subtilis se tento protein označuje RTP.[4]

Reference

  1. ↑ http://www.ebi.ac.uk/pdbe-srv/view/entry/1ecr/summary
  2. ↑ a b NEYLON, Cameron, Andrew V Kralicek, Thomas M Hill, Nicholas E Dixon. Replication termination in Escherichia coli: structure and antihelicase activity of the Tus-Ter complex. Microbiology and Molecular Biology Reviews: MMBR. 2005-09, roč. 69, čís. 3, s. 501–526. Dostupné online [cit. 2009-09-22]. ISSN 1092-2172. DOI 10.1128/MMBR.69.3.501-526.2005. 
  3. ↑ RÉDEI, George P. Encyclopedia of Genetics, Genomics, Proteomics, and Informatics. 3rd Edition. vyd. [s.l.]: Springer, 2008. ISBN 978-1-4020-6753-2. 
  4. ↑ Bacillus: cellular and molecular biology; Od autorů: Peter Graumann

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