SELT
Selenoprotein T , znan i kao SELT , jest protein koji je kod ljudi kodiran genom SELT sa hromosoma 3 .[ 5] [ 6] [ 7]
Aminokiselinska sekvenca
Dužina polipeptidnog lanca je 195 aminokiselina , а molekulska težina 22.324 Da .[ 8]
10 20 30 40 50 MRLLLLLLVA ASAMVRSEAS ANLGGVPSKR LKMQYATGPL LKFQICVSG YRRVFEEYMR VISQRYPDIR IEGENYLPQP IYRHIASFLS VFKLVLIGLI IVGKDPFAFF GMQAPSIWQW GQENKVYACM MVFFLSNMIE NQCMSTGAFE ITLNDVPVWS KLESGHLPSM QQLVQILDNE MKLNVHMDSI PHHRS
Gen
Selenocistein je kodiran UGA kodonom koji normalno signalizira završetak translacije. 3' UTR gena selenoproteina ima zajedničku strukturu petlje i drškre, sekvencu insercije sek (SECIS), koja je neophodna za prepoznavanje UGA kao Sec kodona, a ne kao stop signala .[ 7]
Struktura proteina
Selenoprotein T ima selenocisteinske (Sec) ostatke na aktivnom mjestu .
References
^ a b c GRCh38: Ensembl release 89: ENSG00000198843 - Ensembl , maj 2017^ a b c GRCm38: Ensembl release 89: ENSMUSG00000075700 - Ensembl , maj 2017^ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ Kryukov GV, Kryukov VM, Gladyshev VN (novembar 1999). "New mammalian selenocysteine-containing proteins identified with an algorithm that searches for selenocysteine insertion sequence elements" . J. Biol. Chem . 274 (48): 33888–97. doi :10.1074/jbc.274.48.33888 PMID 10567350 . ^ Kryukov GV, Castellano S, Novoselov SV, Lobanov AV, Zehtab O, Guigó R, Gladyshev VN (maj 2003). "Characterization of mammalian selenoproteomes" . Science . 300 (5624): 1439–43. Bibcode :2003Sci...300.1439K . doi :10.1126/science.1083516 . PMID 12775843 . S2CID 10363908 . ^ a b "Entrez Gene: SELT selenoprotein T" .^ "UniProt, P62341" (jezik: engleski). Pristupljeno 9. 11. 2021 .
Dopunska literatura
Otsuki T, Ota T, Nishikawa T, et al. (2007). "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries" . DNA Res . 12 (2): 117–26. doi :10.1093/dnares/12.2.117 PMID 16303743 . Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)" . Genome Res . 14 (10B): 2121–7. doi :10.1101/gr.2596504 . PMC 528928 PMID 15489334 . Clark HF, Gurney AL, Abaya E, et al. (2003). "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment" . Genome Res . 13 (10): 2265–70. doi :10.1101/gr.1293003 . PMC 403697 PMID 12975309 . Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences" . Proc. Natl. Acad. Sci. U.S.A . 99 (26): 16899–903. doi :10.1073/pnas.242603899 PMC 139241 PMID 12477932 . Yu W, Andersson B, Worley KC, et al. (1997). "Large-scale concatenation cDNA sequencing" . Genome Res . 7 (4): 353–8. doi :10.1101/gr.7.4.353 . PMC 139146 PMID 9110174 . Andersson B, Wentland MA, Ricafrente JY, et al. (1996). "A "double adaptor" method for improved shotgun library construction". Anal. Biochem . 236 (1): 107–13. doi :10.1006/abio.1996.0138 . PMID 8619474 .
