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RPS27
Dostupne strukture
PDB	Pretraga ortologa: PDBe RCSB
Spisak PDB ID kodova
	4UG0, 4V6X, 5A2Q, 5AJ0, 4KZY, 3J7R, 4D61, 4KZX, 4D5L, 5FLX, 4UJD, 3J7P, 4KZZ, 4UJE, 4UJC
Identifikatori
Aliasi	RPS27
Vanjski ID-jevi	OMIM: 603702 MGI: 1915191 HomoloGene: 803 GeneCards: RPS27
Lokacija gena (čovjek)
Hrom.	Hromosom 1 (čovjek)
Kraj	153,992,155 bp
Lokacija gena (miš)
Hrom.	Hromosom 9 (miš)
Kraj	66,856,798 bp
Obrazac RNK ekspresije
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• vezivanje sa DNK; • structural constituent of ribosome; • vezivanje iona cinka; • vezivanje iona metala; • GO:0001948, GO:0016582 vezivanje za proteine; • vezivanje sa RNK;
Ćelijska komponenta	• citosol; • ribozom; • intracellular anatomical structure; • jedro; • nukleoplazma; • cytosolic small ribosomal subunit; • GO:0097483, GO:0097481 postsynaptic density; • presynapse; • glutamatergic synapse; • GABA-ergic synapse;
Biološki proces	• viral transcription; • SRP-dependent cotranslational protein targeting to membrane; • translational initiation; • ribosomal small subunit assembly; • Nonsens-posredovano propadanje iRNK; • Ćelijska proliferacija; • Biosinteza bjelančevina; • sister chromatid cohesion; • rRNA processing;
	Izvori:Amigo / QuickGO
Ortolozi
	6232
	67941
	ENSG00000177954
	ENSMUSG00000036781
	P42677
	Q6ZWY3
	NM_001030; NM_001349946; NM_001349947
	NM_026467; NM_001311101; NM_001361106
	NP_001021; NP_001336875; NP_001336876
	NP_001298030; NP_080743; NP_001348035
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

40S ribosomni protein S27, poznat i kao metalopan-stimulin 1 ili MPS-1, jest protein koji je kod ljudi kodiran genom RPS27 sa hromosoma 1.^[5]^[6]^[7] Metalopanstimulin je cinkoprstni protein za koji se pretpostavlja da je uključen popravak DNK, kao i onkogenezu.^[8]

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 84 aminokiseline, a molekulska težina 9461 Da.^[9]

C: Cistein

D: Asparaginska kiselina

E: Glutaminska kiselina

F: Fenilalanin

G: Glicin

H: Histidin

I: Izoleucin

K: Lizin

L: Leucin

M: Metionin

N: Asparagin

P: Prolin

Q: Glutamin

R: Arginin

S: Serin

T: Treonin

V: Valin

W: Triptofan

Y: Tirozin

10		20		30		40		50
MPLAKDLLHP		SPEEEKRKHK		KKRLVQSPNS		YFMDVKCPGC		YKITTVFSHA
QTVVLCVGCS		TVLCQPTGGK		ARLTEGCSFR		RKQH

Funkcija

Ribosomi, organele koje kataliziraju sintezu proteina, sastoje se od male podjedinice 40S i velike podjedinice 60S. Ove podjedinice zajedno sastoje se od 4 tipa RNK i približno 80 strukturno različitih proteina. Ovaj gen kodira ribosomski protein koji je komponenta podjedinice 40S. Protein pripada porodici ribosomskih proteina S27E. Sadrži domen C4 tipa cinkovog prsta koji se može vezati za cink. Pokazalo se da se kodirani protein može vezati za nukleinske kiseline. Nalazi se u citoplazmi, kao ribosomska komponenta, ali je takođe otkriven u jedru. Studije na pacovima pokazuju da se ribosomni protein S27 nalazi u blizini ribosomnog proteina S18, u podjedinici 40S i da je kovalentno vezan za faktor inicijacije translacije eIF3. Kao što je tipski za gene koji kodiraju ribosomske proteine, postoji više prerađenih pseudogena ovog gena dispergiranih širom genoma.^[7]

Klinički značaj

Njegova ekspresija povećana je kod nekoliko tipova malignosti, a zabilježeno je da nivoi MPS-a opadaju sa liječenjem nekih karcinoma. Također je korišćen kao meta za neke hemoterapije, koje imaju za cilj da helirajući izvuku cink iz motiva cinkovog prsta MPS-a, čineći ga neaktivnim. Ove terapije su se pokazale kao obećavajuće za liječenje raka u laboratorijskim eksperimentima i nekim ograničenim kliničkim ispitivanjima. Karcinom glave i vrata koji je transficiran radi prekomjerne ekspresije ovog proteina pokazao je potisnut rast.^[8]

Reference

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000177954 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000036781 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Tsui SK, Lee SM, Fung KP, Waye MM, Lee CY (Oct 1996). "Primary structures and sequence analysis of human ribosomal proteins L39 and S27". Biochemistry and Molecular Biology International. 40 (3): 611–6. doi:10.1080/15216549600201203. PMID 8908372. S2CID 7909023.
^ Fernandez-Pol JA, Klos DJ, Hamilton PD (Oct 1993). "A growth factor-inducible gene encodes a novel nuclear protein with zinc finger structure". The Journal of Biological Chemistry. 268 (28): 21198–204. doi:10.1016/S0021-9258(19)36910-8. PMID 8407955.
^ ^a ^b "Entrez Gene: RPS27 ribosomal protein S27 (metallopanstimulin 1)".
^ ^a ^b Fernandez-Pol JA (1996). "Metallopanstimulin as a novel tumor marker in sera of patients with various types of common cancers: implications for prevention and therapy". Anticancer Research. 16 (4B): 2177–85. PMID 8694540.
^ "UniProt, P42677" (jezik: engleski). Pristupljeno 18. 12. 2021.

Dopunska literatura

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Stack BC, Hollenbeak CS, Lee CM, Dunphy FR, Lowe VJ, Hamilton PD (decembar 2004). "Metallopanstimulin as a marker for head and neck cancer". World Journal of Surgical Oncology. 2: 45. doi:10.1186/1477-7819-2-45. PMC 544581. PMID 15598348.
Lee WJ, Keefer K, Hollenbeak CS, Stack BC (Oct 2004). "A new assay to screen for head and neck squamous cell carcinoma using the tumor marker metallopanstimulin". Otolaryngology–Head and Neck Surgery. 131 (4): 466–71. doi:10.1016/j.otohns.2004.03.011. PMID 15467619. S2CID 22629299.
Wool IG, Chan YL, Glück A (1996). "Structure and evolution of mammalian ribosomal proteins". Biochemistry and Cell Biology. 73 (11–12): 933–47. doi:10.1139/o95-101. PMID 8722009.
Fernandez-Pol JA, Klos DJ, Hamilton PD (Aug 1994). "Metallopanstimulin gene product produced in a baculovirus expression system is a nuclear phosphoprotein that binds to DNA". Cell Growth & Differentiation. 5 (8): 811–25. PMID 7986747.
Vladimirov SN, Ivanov AV, Karpova GG, Musolyamov AK, Egorov TA, Thiede B, Wittmann-Liebold B, Otto A (Jul 1996). "Characterization of the human small-ribosomal-subunit proteins by N-terminal and internal sequencing, and mass spectrometry". European Journal of Biochemistry. 239 (1): 144–9. doi:10.1111/j.1432-1033.1996.0144u.x. PMID 8706699.
Kenmochi N, Kawaguchi T, Rozen S, Davis E, Goodman N, Hudson TJ, Tanaka T, Page DC (maj 1998). "A map of 75 human ribosomal protein genes". Genome Research. 8 (5): 509–23. doi:10.1101/gr.8.5.509. PMID 9582194.
Yoshihama M, Uechi T, Asakawa S, Kawasaki K, Kato S, Higa S, Maeda N, Minoshima S, Tanaka T, Shimizu N, Kenmochi N (Mar 2002). "The human ribosomal protein genes: sequencing and comparative analysis of 73 genes". Genome Research. 12 (3): 379–90. doi:10.1101/gr.214202. PMC 155282. PMID 11875025.
Atsuta Y, Aoki N, Sato K, Oikawa K, Nochi H, Miyokawa N, Hirata S, Kimura S, Sasajima T, Katagiri M (Aug 2002). "Identification of metallopanstimulin-1 as a member of a tumor associated antigen in patients with breast cancer". Cancer Letters. 182 (1): 101–7. doi:10.1016/S0304-3835(02)00068-X. PMID 12175529.
Martin-Lluesma S, Stucke VM, Nigg EA (Sep 2002). "Role of Hec1 in spindle checkpoint signaling and kinetochore recruitment of Mad1/Mad2". Science. 297 (5590): 2267–70. Bibcode:2002Sci...297.2267M. doi:10.1126/science.1075596. PMID 12351790. S2CID 7879023.
Liu ST, Chan GK, Hittle JC, Fujii G, Lees E, Yen TJ (Apr 2003). "Human MPS1 kinase is required for mitotic arrest induced by the loss of CENP-E from kinetochores". Molecular Biology of the Cell. 14 (4): 1638–51. doi:10.1091/mbc.02-05-0074. PMC 153128. PMID 12686615.
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Bhonde MR, Hanski ML, Budczies J, Cao M, Gillissen B, Moorthy D, Simonetta F, Scherübl H, Truss M, Hagemeier C, Mewes HW, Daniel PT, Zeitz M, Hanski C (Mar 2006). "DNA damage-induced expression of p53 suppresses mitotic checkpoint kinase hMps1: the lack of this suppression in p53MUT cells contributes to apoptosis". The Journal of Biological Chemistry. 281 (13): 8675–85. doi:10.1074/jbc.M511333200. PMID 16446370.
Wang YW, Qu Y, Li JF, Chen XH, Liu BY, Gu QL, Zhu ZG (Aug 2006). "In vitro and in vivo evidence of metallopanstimulin-1 in gastric cancer progression and tumorigenicity". Clinical Cancer Research. 12 (16): 4965–73. doi:10.1158/1078-0432.CCR-05-2316. PMID 16914586.
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Vanjski linkovi

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000177954 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000036781 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid8908372-5] Tsui SK, Lee SM, Fung KP, Waye MM, Lee CY (Oct 1996). "Primary structures and sequence analysis of human ribosomal proteins L39 and S27". Biochemistry and Molecular Biology International. 40 (3): 611–6. doi:10.1080/15216549600201203. PMID 8908372. S2CID 7909023.

[pmid8407955-6] Fernandez-Pol JA, Klos DJ, Hamilton PD (Oct 1993). "A growth factor-inducible gene encodes a novel nuclear protein with zinc finger structure". The Journal of Biological Chemistry. 268 (28): 21198–204. doi:10.1016/S0021-9258(19)36910-8. PMID 8407955.

[entrez-7] "Entrez Gene: RPS27 ribosomal protein S27 (metallopanstimulin 1)".

[Fernandez-Pol_1996-8] Fernandez-Pol JA (1996). "Metallopanstimulin as a novel tumor marker in sera of patients with various types of common cancers: implications for prevention and therapy". Anticancer Research. 16 (4B): 2177–85. PMID 8694540.

[UniProt-9] "UniProt, P42677" (jezik: engleski). Pristupljeno 18. 12. 2021.

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