CCT8L1P

CCT8L1P
Identifikatori
AliasiCCT8L2
Vanjski ID-jeviMGI: 2685289 HomoloGene: 40943 GeneCards: CCT8L2
Lokacija gena (čovjek)
Hromosom 22 (čovjek)
Hrom.Hromosom 22 (čovjek)[1]
Hromosom 22 (čovjek)
Genomska lokacija za CCT8L1P
Genomska lokacija za CCT8L1P
Bend22q11.1Početak16,590,751 bp[1]
Kraj16,592,810 bp[1]
Lokacija gena (miš)
Hromosom 5 (miš)
Hrom.Hromosom 5 (miš)[2]
Hromosom 5 (miš)
Genomska lokacija za CCT8L1P
Genomska lokacija za CCT8L1P
Bend5|5 B1Početak25,721,065 bp[2]
Kraj25,723,025 bp[2]
Ontologija gena
Molekularna funkcija• nucleotide binding
• calcium-activated potassium channel activity
• anion channel activity
• ATP binding
• protein folding chaperone activity
• unfolded protein binding
Ćelijska komponenta• citoplazma
• TRiC
Biološki proces• potassium ion transmembrane transport
• 'de novo' protein folding
• chaperone-mediated protein folding
• inorganic anion transport
Izvori:Amigo / QuickGO
Ortolozi
VrsteČovjekMiš
Entrez

150160

242891

Ensembl

ENSG00000198445

ENSMUSG00000038044

UniProt

Q96SF2

Q80YT3

RefSeq (mRNK)

NM_014406

NM_198621

RefSeq (bjelančevina)

NP_055221

NP_941023

Lokacija (UCSC)Chr 22: 16.59 – 16.59 MbChr 5: 25.72 – 25.72 Mb
PubMed pretraga[3][4]
Wikipodaci
Pogledaj/uredi – čovjekPogledaj/uredi – miš

CESK1/CCT8L1P kodiran je iz genakog lokusa na hromosomu 22.[5] T-kompleksni protein nalazi se u sljedećim uzorcima: serum, krv, plazma, supernatant kultura ćelija su i ostalim srodnim supernatantima i tkivima.[6][7]

Enzimski povezani imunosorbentni testovi za ispitivanje dizajnirani su za kvantitativno mjerenje analita u širokom spektru uzoraka. Kako današnji naučnici zahtijevaju visokokvalitetne konzistentne podatke za časopise s velikim utjecajem, razvijen je niz osjetljivih, brzih i pouzdanih ELISA testova.

Za njegovo određivanje koristi se kompetitivni-ELISA metod. Mikrolitarska ploča prethodno se presvuče CESK1/CCT8L1P / T-kompleksom proteinske 1 tetolike podjedinice 1. Tokom reakcije, CESK1/CCT8L1P / T-kompleksnog proteina 1 u uzorku ili standardu se takmiči sa fiksnom količinom CESK1 / CCT8L1P / pretpostavljenog T-kompleksnog proteina 1 na nosaču čvrste faze za lokacije, specifične za CESK1/CCT8L1P/T-kompleks proteina 1, podjedinice nalik na teta 1. Višak konjugata i nevezani uzorak ili standard ispere se sa ploče, a HRP-streptavidin (SABC) doda se u svaku mikroploču i inkubira. Zatim se u svaku jažicu doda rastvor TMB supstrata. Reakcija enzim-supstrat završava se dodavanjem otopine sumporne kiseline i promjena boje mjeri se spektrofotometrijski, na talasnoj dužini od 450 nm. Zatim se uspoređivanjem određuje koncentracija CESK1/CCT8L1P/putativnog T-kompleksa proteina 1, podjedinice teta-1 u uzorcima, na standardnoj krivulji.

Također pogledajte

Reference

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000198445 - Ensembl, maj 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000038044 - Ensembl, maj 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ https://www.CCT8L2[mrtav link] Gene - GeneCards | TCPQM Protein | TCPQM Antibody
  6. ^ https://www.raybiotech.com/Human[mrtav link] CESK1 monoclonal antibody (100 ug) [101-10285] (raybiotech.com): Anti-Human CESK1, publisher: RayBiotech
  7. ^ https://www.genetex.com/Product/Detail/CESK1/Anti-CESK1[mrtav link] antibody (GTX117858), GeneTex CESK1 Monoclonal Antibody (2C9) Thermo Fisher Scientific https://www/CESK1[mrtav link] Antibody (H00150160-M12) (thermofisher.com)

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