Will Chambers' senior career began with the Melbourne Storm in the NRL. His usual position is as a centre or wing. Chambers also played for Brisbane Norths in the Queensland Cup. When Matt King departed the Melbourne Storm after their 2007 NRL Grand Final victory, he nominated rookie Melbourne Storm centre Will Chambers as the man to take his place.[9]
Chambers final game for Melbourne in this period was the 2009 NRL Grand Final win against the Parramatta Eels. This premiership title was later stripped due to salary cap breaches by the Melbourne Storm.[10]
2012 - 2019: Melbourne Storm
He re-joined the Melbourne Storm in 2012, and played his first game on 25 March 2012 against the Sydney Roosters, in which he scored a try. A successful return was exclaimed by scoring a hat-trick against New Zealand Warriors.
Chambers was called into the Queensland team for the 2014 State of Origin series as 18th man for the first two games. However, with Brent Tate being injured in game two, it left vacant the wing position for Chambers to make his Origin debut at Suncorp Stadium in Game Three.
In the 2018 NRL season, Melbourne finished second on the table and reached the 2018 NRL Grand Final against the Sydney Roosters. Melbourne were defeated 21–6 in the final and Chambers was involved in an incident with Roosters player Latrell Mitchell during the match where Chambers was tackled into touch and then pushed into the advertising board by Mitchell. This was a culmination of a rivalry between the two players which had started earlier in the year during the 2018 State of Origin series.[12]
In the 2019 NRL season, Melbourne won the Minor Premiership after a dominant season but were defeated by the Sydney Roosters at the Sydney Cricket Ground. This would be Chambers final game for Melbourne.[13]
In round 15 Chambers scored his first try for Cronulla against North Queensland. Cronulla-Sutherland would go on the win the game 26-24 in a tight finish.[16]
On 10 August, Chambers was demoted from the Cronulla side by interim head coach Josh Hannay. Hannay was reportedly unhappy with Chambers on-field sledging where in consecutive weeks he sledged Manly's Dylan Walker and then against New Zealand Warriors player Kane Evans.[17]
Rugby Union
2010 - 2011: Queensland Reds
Chambers switched to rugby union from 2010 after signing a 2-year deal with the Queensland Reds to play in the Super 14.[18] However, following a disappointing 2011 season - where he only started six of the Reds' 18 matches - Chambers was released by the club after missing a place in their thirty-man 2012 squad.[19] He was named on the bench for the Reds Super 15 final victory over the Crusaders in 2011 – which featured fellow league-turned-union player Sonny Bill Williams on the opposing side – but did not play in the match.[19]
2011: Munster
Chambers signed for Pro12 side and former European Champions Munster in September 2011 on a short-term deal.[20] He arrived to join up with Munster in October 2011.[21] He played for Munster A against Connacht A on 21 October 2011.[22] He made his full Munster debut against Aironi on 28 October 2011.[23] He made his Heineken Cup debut for Munster against Northampton Saints on 12 November 2011.[24][25] Chambers scored his first try for Munster exactly a week later, in their second Pool One game away to Castres Olympique.[26] He was named in the Munster A team to play Ulster Ravens in the British and Irish Cup on 20 January 2012.[27] This was announced to be Chambers last appearance for Munster, as he was returning to Australia to take up a contract with rugby league team Melbourne Storm.[28] Munster A won the game 9–20, securing a spot in the semi-finals.[29] In his four months with Munster, Chambers made 8 appearances for the senior Munster team, including 3 Heineken Cup caps, and scored 1 try. He also made 3 appearances for Munster A.
2020: Suntory Sungoliath
Following the conclusion of the 2019 Melbourne Storm season, Chambers was honoured at the club's annual awards night as one of the departing players, confirming rumours that he would be leaving the NRL.[30] Chambers would later be unveiled by Suntory Sungoliath, who also had former Queensland Reds Sam Talakai, Hendrik Tui & Samu Kerevi on their roster.