Nicoletta Sacchi

Nicoletta Sacchi
Born(1949-09-20)September 20, 1949
Milan, Italy
NationalityItalian
Alma materUniversity of Milan
Known forAcid guanidinium thiocyanate-phenol-chloroform extraction
Scientific career
FieldsBiochemistry, Oncology
InstitutionsRoswell Park Comprehensive Cancer Center

Nicoletta Sacchi (born August 20, 1949) is an Italian professor of oncology at the Roswell Park Comprehensive Cancer Center.[1] She is the co-discoverer of the acid guanidinium thiocyanate-phenol-chloroform extraction method to extract RNA from biological samples with Trizol.[2] As a consequence of this groundbreaking discovery she is now considered the most cited woman scientist in the world.[3]

Career

Sacchi was born in Milan and studied for her PhD at the University of Milan in 1972, investigating the genetics of yeast. She remained within the fields of genetics for her postdoctoral training at the same University and Erasmus University Rotterdam.[2][4][1] Sacchi worked on the generation of antibody repertoires by the TdT DNA polymerase in the lab of Georges Köhler at the Basel Institute for Immunology.[2]

She moved to the US in 1982 and switched to cancer research, focussing on the analysis of the genetics of patient samples at National Cancer Institute.[4] This required a high yield technique to extract RNA from scarce paediatric leukemia samples, so her and colleague Piotr Chomczynski developed a new method to extract the nucleic acid.[5] Since the publication of the technique in 1987 it has become ubiquitous in biological research, earning the paper the accolade of being one of the most cited in history.[3][6]

Sacchi went back to the University of Milan in 1991 and was promoted to professor.[4] Unsatisfied with research in Italy she spent time back in the US as a visiting professor at the Sydney Kimmel Comprehensive Cancer Center at Johns Hopkins University.[2][4] In 2003 she joined the Roswell Park Comprehensive Cancer Center where she continues to study the role of genetics and the epigenome on cancer development.[2][3]

Sacchi has previously criticised the culture of science in her home nation of Italy, describing the system as “fairly corrupt” and ignorant of the achievements of female scientists. She believes that this has led to brain drain of trained researchers.[3]

Accolades

In 2001 Sacchi was proclaimed a “Highly Cited Researcher in Biology and Biochemistry” by the Institute for Scientific Information in Philadelphia. In 2007 the Italian President awarded Sacchi with the Commander of Merit.[2]

References

  1. ^ a b "Nicoletta Sacchi, la sua vita e i suoi studi". Video: ultime notizie - Corriere TV (in Italian). Retrieved 2020-02-29.
  2. ^ a b c d e f "Nicoletta Sacchi". Roswell Park Comprehensive Cancer Center. Retrieved 2020-02-28.
  3. ^ a b c d Guardian Staff (2003-11-18). "World class: Nicoletta Sacchi view from Italy". The Guardian. ISSN 0261-3077. Retrieved 2020-02-28.
  4. ^ a b c d "Oral History | Life in Science | Women in Science | Nicoletta Sacchi on Scientific Career". library.cshl.edu. Retrieved 2020-02-29.
  5. ^ Chomczynski, Piotr; Sacchi, Nicoletta (1987-04-01). "Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction". Analytical Biochemistry. 162 (1): 156–159. doi:10.1016/0003-2697(87)90021-2. ISSN 0003-2697.
  6. ^ Chomczynski, Piotr; Sacchi, Nicoletta (August 2006). "The single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction: twenty-something years on". Nature Protocols. 1 (2): 581–585. doi:10.1038/nprot.2006.83. ISSN 1750-2799. PMID 17406285.

Read other articles:

Bad Thief, Good Thief

Bad Thief, Good ThiefPoster promosiGenreHukumDramaPembuatLee Jae-Dong[1]Ditulis olehSon Young-mokCha Yi-young[1]SutradaraOh Kyung-hoonJang Joon-ho[1]PemeranJi Hyun-wooSeohyunKim Ji-hoonLim Ju-eunNegara asalKorea SelatanBahasa asliKoreaJmlh. episode50ProduksiProduser eksekutifLee Jae-dong[1]ProduserKim Jin-cheonDurasi70 menitRumah produksiMay Queen PicturesDistributorMunhwa Broadcasting CorporationRilis asliJaringanMBC TVFormat gambar1080i (HDTV)Format audio2 c...

 

Saxons

Germanic tribes from the North German Plain Saxon redirects here. For other uses, see Saxon (disambiguation). SaxonsSahsonSpread of Angles (orange) and Saxons (blue) around 500 ADRegions with significant populationsOld Saxony, Jutland, Frisia, Heptarchy (England)LanguagesOld Saxon, Old EnglishReligionOriginally Germanic and Anglo-Saxon paganism, later ChristianityRelated ethnic groupsAnglo-Saxons, Angles, Frisii, Jutes The Saxons[1] were a group of Germanic[2] peoples who...

 

Bus sekolah

Bus sekolah di IndonesiaBeberapa siswa sekolah dan bus sekolah yang dimiliki persatuan pegawai perkebunan di Pangalengan, Bandung pada masa Hindia Belanda, 1938Bus sekolah di Jakarta, 2014 Bus sekolah adalah jenis bus angkutan untuk siswa sekolah yang dimiliki, disewa, dikontrak, atau dioperasikan oleh sekolah atau perusahaan otobus. Bus jenis ini biasanya digunakan untuk mengangkut siswa ke dan dari sekolah atau kegiatan terkait sekolah. Ciri-ciri pelayanan bus sekolah di Indonesia diatur be...

العلاقات الغامبية القطرية

العلاقات الغامبية القطرية غامبيا قطر   غامبيا   قطر تعديل مصدري - تعديل   العلاقات الغامبية القطرية هي العلاقات الثنائية التي تجمع بين غامبيا وقطر.[1][2][3][4][5] مقارنة بين البلدين هذه مقارنة عامة ومرجعية للدولتين: وجه المقارنة غامبيا قطر المس�...

 

Mahatma Gandhi

Disambiguazione – Gandhi rimanda qui. Se stai cercando altri significati, vedi Gandhi (disambigua). Questa voce o sezione sull'argomento biografie è priva o carente di note e riferimenti bibliografici puntuali. Commento: in numerosi passaggi della biografia non sono presenti fonti: non si riesce a capire da dove siano state estratte; inoltre le fonti per una personalità di una simile caratura non sono sufficienti. Sebbene vi siano una bibliografia e/o dei collegamenti estern...

 

Overwatch (permainan video)

Overwatch Gambar Edisi Asli permainan, menampilkan karakter TracerPublikasiWindows, PS4,[1][2] Xbox One24 Mei, 2016Nintendo Switch15 Oktober, 2019[3][4][5]Versi 1.74.0.0.104319 (6 September 2022) GenrePenembak orang pertamaKarakterHanzo (en), Tracer, Torbjörn (en), Brigitte (en), Winston (en), Widowmaker (en), Sombra (en), Wrecking Ball (en), D.Va (en), Symmetra (en), Ana (en), Mercy (en), Roadhog (en), Mei (en), Pharah (en), Doomfist (en), Junkrat (en...

Lake Apopka

Lake in the state of Florida, United States This article has multiple issues. Please help improve it or discuss these issues on the talk page. (Learn how and when to remove these template messages) This article needs to be updated. Please help update this article to reflect recent events or newly available information. (November 2010) This article needs additional citations for verification. Please help improve this article by adding citations to reliable sources. Unsourced material may be c...

 

Kyzyl

Kyzyl КызылKotaTranskripsi Other • TuvanКызы́л BenderaLambang kebesaranPeta lokasi Kyzyl KyzylLokasi KyzylTampilkan peta RusiaKyzylKyzyl (Rusia)Tampilkan peta RusiaKoordinat: 51°43′N 94°27′E / 51.717°N 94.450°E / 51.717; 94.450Koordinat: 51°43′N 94°27′E / 51.717°N 94.450°E / 51.717; 94.450NegaraRusiaSubjek federalTuvaPendirian1914Status sejak1914Pemerintahan • Wali kotaVladislav Tovarischta...

 

Echo (satelit)

Satelit ECHO dikenal juga sebagai satelit HIRO. HIRO ialah satelit komunikasi pasif pertama Amerika Serikat. Terbuat dari balon metal, berdiameter 30 meter (HIRO 1) dan 42 meter (HIRO 2). Berturut-turut, kedua satelit itu diluncurkan pada tahun 1960 dan 1964 oleh NASA. Echo 1 digelembungkan penuh di hanggar US Navy di Weeksville, North Carolina. Hiro 1 merupakan satelit komunikasi pasif: berfungsi sebagai pemantul, bukan pemancar. Setelah ditempatkan di orbit bumi rendah, sinyal akan dikirim ...

Eucalyptus occidentalis

Species of eucalyptus Flat-topped yate Scientific classification Kingdom: Plantae Clade: Tracheophytes Clade: Angiosperms Clade: Eudicots Clade: Rosids Order: Myrtales Family: Myrtaceae Genus: Eucalyptus Species: E. occidentalis Binomial name Eucalyptus occidentalisEndl. Synonyms Eucalyptus agnata Domin Eucalyptus occidentalis var. occidentalis Eucalyptus occidentalis, commonly known as the flat topped yate or the swamp yate, is a tree that is native to Western Australia.[1] The ...

 

Komando Distrik Militer 1411

Komando Distrik Militer 1411/BulukumbaLambang Kodim 1411/BulukumbaNegara IndonesiaAliansi Korem 141/ToddopuliCabang TNI Angkatan DaratTipe unitKodim Tipe BPeranSatuan TeritorialBagian dari Kodam XIV/HasanuddinMakodimJl. Jenderal Ahmad Yani, Kelurahan Ela-Ela, Kecamatan Ujung Bulu, Kabupaten Bulukumba, Sulawesi SelatanPelindungTentara Nasional IndonesiaMotoKonjo: ᨆᨒᨗ ᨔᨗᨄᨑᨄᨙ ᨈᨒ ᨔᨗᨄᨖᨘᨕMali' Siparappe, Tallang SipahuaTerbawa arus air, maka bahu-membahu salin...

 

Avianca (El Salvador)

Este artículo o sección se encuentra desactualizado.La información suministrada ha quedado obsoleta o es insuficiente, pero puede consultarse actualizada en tema del artículo.Este aviso fue puesto el 4 de enero de 2024. Avianca El Salvador El cielo es de todos Airbus A320 de Avianca El Salvador (2013). IATATA OACITAI IndicativoTACA Fundación 1931 (93 años) (como Transportes Aéreos Centroamericanos TACA)2013 (11 años) (como Avianca El Salvador)Aeropuerto principal Aeropuerto...

National Socialist Party of America

Not to be confused with the American Nazi Party. Political party in United States National Socialist Party of America AbbreviationNSPALeaderFrank Collin (1970–1977)Harold Covington (1977–1981)Founded1970; 54 years ago (1970)Dissolved1981; 43 years ago (1981)Split fromAmerican Nazi PartyHeadquartersChicago, IllinoisRaleigh, North CarolinaIdeologyNeo-NazismWhite supremacismWhite nationalismAntisemitismPolitical positionFar-rightPolitics of U...

 

MV Beluga Nomination incident

2011 conflict MV Beluga Nomination incidentPart of Piracy in Somalia, Operation Ocean Shield, Operation Enduring Freedom - Horn of AfricaHDMS Esbern SnareDate22–26 January 2011Locationoff Somalia, Indian OceanResult Pirate victory, rescue attempt failed. 2 civilians rescuedBelligerents  Denmark Seychelles Somali piratesStrength 1 support ship (frigate)1 patrol boat 2 freightersCasualties and losses Unknown 1–2 killed1 freighter damaged 4 civilians killed vteOperation Ocean Shiel...

 

Self-adhesive stamp

Self-adhesive postage stamp This article needs additional citations for verification. Please help improve this article by adding citations to reliable sources. Unsourced material may be challenged and removed.Find sources: Self-adhesive stamp – news · newspapers · books · scholar · JSTOR (March 2020) (Learn how and when to remove this message) 2005 self-adhesive stamp of Deutsche Post. (450 years after Augsburger Religionsfrieden) A self-adhesive stamp...

Mano (stone)

Hand-held stone tool used with a metate or quern to process or grind food by hand Native American manos from Arizona. A mano (Spanish for hand) is a ground stone tool used with a metate to process or grind food by hand.[1] It is also known as metlapil, a term derived from Nahuatl.[2] History Manos were used in prehistoric times to process wild seeds, nuts, and other food, generally used with greater frequency in the Archaic period, when people became more reliant upon local wi...

 

الحبيب التوهامي

يفتقر محتوى هذه المقالة إلى الاستشهاد بمصادر. فضلاً، ساهم في تطوير هذه المقالة من خلال إضافة مصادر موثوق بها. أي معلومات غير موثقة يمكن التشكيك بها وإزالتها. (ديسمبر 2018) الحبيب التوهامي وزير الصحة العمومية التونسي في المنصب14 أكتوبر 1983 – 20 يناير 1984 (3 أشهرٍ و6 أيامٍ) الرئيس ال�...

 

1953 Philippine Senate election

1953 Philippine Senate election ← 1951 November 10, 1953 1955 → 8 (of the 24) seats in the Senate13 seats needed for a majority   First party Second party   Leader Eulogio Rodriguez Quintin Paredes Party Nacionalista Liberal Seats before 11 11 Seats after 13 7 Seat change 2 4 Popular vote 9,813,166 8,861,244 Percentage 39.8% 36.0% Swing 19.2% 3.0%   Third party Fourth party   Leader Fernando Lopez Lorenzo Tañada Party Democratic ...

Beta-Nitropropionic acid

β-Nitropropionic acid Names Preferred IUPAC name 3-Nitropropanoic acid Other names 3-Nitropropionic acid; β-Nitropropanoic acid; BPA; 3-NPA Identifiers CAS Number 504-88-1 Y 3D model (JSmol) Interactive image ChemSpider 1615 ECHA InfoCard 100.007.276 PubChem CID 1678 UNII QY4L0FOX0D Y CompTox Dashboard (EPA) DTXSID1020982 InChI InChI=1S/C3H5NO4/c5-3(6)1-2-4(7)8/h1-2H2,(H,5,6)Key: WBLZUCOIBUDNBV-UHFFFAOYSA-N SMILES C(C[N+](=O)[O-])C(=O)O Properties Chemical formula C3H5NO4 Mo...

 

Common dab

Species of fish Common dab Conservation status Least Concern  (IUCN 3.1)[1] Scientific classification Domain: Eukaryota Kingdom: Animalia Phylum: Chordata Class: Actinopterygii Order: Pleuronectiformes Family: Pleuronectidae Genus: Limanda Species: L. limanda Binomial name Limanda limanda(Linnaeus, 1758) Common dab range.[2] Synonyms Pleuronectes limanda Linnaeus, 1758 Liopsetta limanda (Linnaeus, 1758) Pleuronectes limandula Bonnaterre, 1788 Limanda vulgaris Gottsch...