Provine was born in Deadwood in southwestern South Dakota, to William and Irene Provine, but grew up in Seattle, Washington, where her parents ran a nightclub.[1][2] She attended the University of Washington in Seattle, from which she graduated with a degree in Theater Arts in 1957.[3] While there, she joined the women's fraternity Alpha Gamma Delta.[4] In Washington, she handed out prizes for a quiz program on a local television station until she was hired by Warner Bros. at $500 per week.[5] In Hollywood, she starred in the titular role as the cigar-chomping, machine-gun firing heroine of the 1958 film The Bonnie Parker Story directed by William Witney. That same year, she performed in a credited walk-on part in the NBCWestern television series Wagon Train, in the episode "The Marie Dupree Story." In 1959, she was in the cast of The 30 Foot Bride of Candy Rock, which was Lou Costello's last screen appearance.[6] In that same year she again appeared in Wagon Train in the episode "Matthew Lowry Story", this time having a part that ran the full episode.[7]
On January 3, 1959, Provine appeared as Laura Winfield in the episode "The Bitter Lesson" of the NBC Western series Cimarron City. Laura Winfield is a newly arrived schoolteacher with false credentials who is plotting with a male companion to rob a stage shipment of gold, but not before Deputy Sheriff Lane Temple (series star John Smith) falls in love with her. Dan Blocker and Gregg Palmer also appear in this episode as interested suitors of the new teacher. A few weeks thereafter, she was cast in a supporting role in the episode "The Giant Killer" of the ABC/Warner Bros. Western series Sugarfoot, with Will Hutchins in the title role.[8]
Provine had a starring role in two ABC/WB series: The Alaskans (1959–1960) starring Roger Moore in which she played Rocky Shaw, and The Roaring Twenties (1960–1962), in which she portrayed dazzling singer/dancer Pinky Pinkham. A profile in Time stated that "It is Dorothy’s oooohing and shimmying that have kept the series afloat."[5] Rex Reason, from Man Without a Gun, co-starred with her in The Roaring Twenties along with Donald May, John Dehner, Mike Road, and Gary Vinson. Provine recorded an album of songs from the show, and had two hit singles in the UK Singles Chart — "Don't Bring Lulu" (number 17 in 1961) and "Crazy Words, Crazy Tune" (number 45 in 1962).[11]
She guest-starred in the 25th episode of The Gallant Men called "Tommy", which aired in March 1963. Her character name was Joyce Adams, a singer who entertained the troops.[9]
In 1968, Provine married the film and television director Robert Day and retired from acting, apart from occasional guest roles on television. About 1990 the couple moved to Bainbridge Island, Washington, where they resided with their son.[12][13] Provine was reclusive in retirement, indulging her love of reading and movies, occasionally driving around the island with her husband.[6]
The Roaring 20's, 1960 Warner Bros.: WM 4035 (W1394). Musical direction by Sandy Courage. Included two songs which were A sides of hit singles: Don't Bring Lulu and Crazy Words - Crazy Tune.
The Vamp of The Roaring 20s - Vol. 2, 1961 Warner Bros.: WM 4053. Musical direction by Sandy Courage.
Oh You Kid!, 1962 Warner Bros.: 1962 Warner Bros.: W 1466. With Joe "Fingers" Carr.