Calu-3

Calu-3 is a human lung cancer cell line commonly used in cancer research and drug development. Calu-3 cells are epithelial and can act as respiratory models in preclinical applications.[1]

Calu-3 cells were first derived in 1975 by Germain Trempe and Jorgen Fogh of the Memorial Sloan Kettering Cancer Center. The cells were isolated from the pleural effusion of a 25-year-old Caucasian male with a lung adenocarcinoma.[citation needed]

The ERBB2 gene is overexpressed in Calu-3 cells, and plays a role in aggressive cancer proliferation

The Calu-3 cell line grows as an adherent monolayer and overexpresses the ERBB2 gene, leading to active ErbB2/Her2. The cells also express CK7, occludin, and E-cadherin.[2] Calu-3 cells also have large amounts of cystic fibrosis transmembrane conductance regulator.[3]

Calu-3 cells are commonly used as both in vitro and in vivo models for drug development against lung cancer.[2] The cells have been used in studies of pulmonary drug delivery, demonstrating a capacity to intake low molecular weight substances.[4] Calu-3 cells have served as respiratory models for air intake and lung injury due to their responsiveness to foreign substances.[1] The Calu-3 cell line has shown to be useful in the study of chloride ion secretion by lung epithelial cells.[3] The cells may be used in high-throughput screening applications focused on barrier integrity and surface protein expression of lung cells.[5]

References

  1. ^ a b Zhu, Yan; et al. (2010). "Cultured Human Airway Epithelial Cells (Calu-3): A Model of Human Respiratory Function, Structure, and Inflammatory Responses". Critical Care Research and Practice. 2010: 1–8. doi:10.1155/2010/394578. PMC 2951077. PMID 20948883.
  2. ^ a b Kreft, Mateja; et al. (10 March 2015). "The characterization of the human cell line Calu-3 under different culture conditions and its use as an optimized in vitro model to investigate bronchial epithelial function". European Journal of Pharmaceutical Sciences. 69: 1–9. doi:10.1016/j.ejps.2014.12.017. PMID 25555374.
  3. ^ a b Shen, BQ; et al. (May 1994). "Calu-3: a human airway epithelial cell line that shows cAMP-dependent Cl- secretion". American Journal of Physiology. 266 (5): 493–501. doi:10.1152/ajplung.1994.266.5.L493. PMID 7515578.
  4. ^ Foster, KA; et al. (4 November 2000). "Characterization of the Calu-3 cell line as a tool to screen pulmonary drug delivery". International Journal of Pharmaceutics. 208 (1): 1–11. doi:10.1016/S0378-5173(00)00452-X. PMID 11064206.
  5. ^ Haghi, Mehra; et al. (11 February 2010). "Time- and passage-dependent characteristics of a Calu-3 respiratory epithelial cell model". Drug Development and Industrial Pharmacy. 36 (10): 1207–1214. doi:10.3109/03639041003695113. PMID 20374185. S2CID 25505730.