The 2016 Aspria Tennis Cup was a professional clay court tennis tournament that took place in Milan from 20–26 June. It was the 11th edition of the tournament and a part of the 2016 ATP Challenger Tour.
The following players received wildcards into the singles main draw:
The following player received entry into the singles main draw as a special exempt:
The following players received entry as alternates:
The following players received entry from the qualifying draw:
