During the 2000–01 German football season, FC Energie Cottbus competed in the Bundesliga.

Cottbus finished 14th in the Bundesliga.

Squad at end of season^[1]

Note: Flags indicate national team as defined under FIFA eligibility rules; some limited exceptions apply. Players may hold more than one non-FIFA nationality.

No. Pos. Nation Player 1 GK  GER Thomas Köhler 2 DF  HUN Vilmos Sebők 3 DF  GER Christian Beeck 4 MF  GER Jörg Scherbe 5 DF  BUL Tsanko Tsvetanov 7 MF  ROU Laurențiu Reghecampf 8 MF  BIH Bruno Akrapović 9 MF  MKD Toni Micevski 10 MF  GER Steffen Heidrich 11 FW  CRO Antun Labak 13 MF  POL Witold Wawrzyczek 14 DF  ALB Rudi Vata 16 MF  BEN Moussa Latoundji

No. Pos. Nation Player 17 DF  HUN János Mátyus 18 FW  POL Andrzej Kobylański 19 MF  MAR Hamid Termina 20 DF  GER René Trehkopf 21 FW  BRA Franklin Bittencourt 22 FW  MAR Mohamed Lakhouil 23 GK  BIH Tomislav Piplica 24 MF  HUN Vasile Miriuță[2] 25 DF  BIH Faruk Hujdurović 30 MF  SWE Jonny Rödlund 31 DF  CAN Kevin McKenna 33 MF  GER Ronny Thielemann 34 FW  GER Sebastian Helbig

Note: Flags indicate national team as defined under FIFA eligibility rules; some limited exceptions apply. Players may hold more than one non-FIFA nationality.

No. Pos. Nation Player 15 FW  HUN Ferenc Horváth (to Maccabi Tel Aviv) 15 FW  ROU Sabin Ilie (to Dinamo Bucharest)

No. Pos. Nation Player 19 FW  GER Toralf Konetzke (to St Pauli)