金胺O

Auramine O
IUPAC名
bis[4-(dimethylamino)phenyl]methaniminium chloride
别名 Basic yellow 2, Pyocatanium aureum, aizen auramine, Pyoktanin Yellow, Canary Yellow, Pyoktanin, or C.I. 41000
识别
CAS号 2465-27-2  checkY
PubChem 17170
ChemSpider 16254
SMILES
 
  • [N@H]=C(c1ccc(N(C)C)cc1)c2ccc(N(C)C)cc2
InChI
 
  • 1/C17H21N3/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4/h5-12,18H,1-4H3
InChIKey JPIYZTWMUGTEHX-UHFFFAOYAY
性质
化学式 C17H22ClN3
摩尔质量 303.83 g·mol−1
熔点 267 °C
危险性
警示术语 R：R22 R24 R40
安全术语 S：S36/37 S45
若非注明，所有数据均出自标准状态（25 ℃，100 kPa）下。

金胺O是一種二芳基甲烷螢光染料，常態下為黃色針狀結晶。極易溶於水，易溶於乙醇。

金胺O可用於抗酸細菌（例如Mycobacterium）, 其方法類似抗酸染色法。[1] 也被當做希夫試劑的螢光版來使用。[2]

在金胺-若丹明染色法中與若丹明B一起用來給結核桿菌染色。[3][4] 同時也可用作防腐劑。

參考文獻

  1. ^ Kommareddi S, Abramowsky C, Swinehart G, Hrabak L. Nontuberculous mycobacterial infections: comparison of the fluorescent auramine-O and Ziehl-Neelsen techniques in tissue diagnosis. Hum Pathol. 1984, 15 (11): 1085–9. PMID 6208117. doi:10.1016/S0046-8177(84)80253-1. 
  2. ^ Khavkin T, Kudryavtseva M, Dragunskaya E, Polotsky Y, Kudryavtsev B. Fluorescent PAS-reaction study of the epithelium of normal rabbit ileum and after challenge with enterotoxigenic Escherichia coli. Gastroenterology. 1980, 78 (4): 782–90. PMID 6986320. 
  3. ^ Truant J, Brett W, Thomas W. Fluorescence microscopy of tubercle bacilli stained with auramine and rhodamine. Henry Ford Hosp Med Bull. 1962, 10: 287–96. PMID 13922644. 
  4. ^ Arrowood M, Sterling C. Comparison of conventional staining methods and monoclonal antibody-based methods for Cryptosporidium oocyst detection. J Clin Microbiol. 1989, 27 (7): 1490–5. PMC 267601 可免费查阅. PMID 2475523. 

外部連結