Viralni vektor

Viralni vektor je oruđe koje se često koristi u molekularnoj biologiji za isporuku genetičkog materijala u ćelije.[1] Ova proces se može vršiti unutar živog organizma (in vivo) ili u ćelijskoj kulturu (in vitro). Virusi su evoluirali specijalizovane molekularne mehanizme za efikasni transport njihovog genoma u ćelije koje inficiraju. Isporuka gena virusima se naziva transdukcija, a inficirane ćelije su trandukovane. Ovaj mehanizam je našao laboratorijsku primenu tokom 1970-tih. Paul Berg je koristio modifikovani SV40 virus koji je sadržao DNK iz bakteriofaga lambda da inficira ćelije bubrega majmuna održavane u kulturi.[2]

Reference

  1. ^ Bruce Alberts; Alexander Johnson; Julian Lewis; Martin Raff; Keith Roberts; Peter Walter (2002). Molecular Biology of the Cell. New York: Garlard Science. ISBN 0815332181. 
  2. ^ Goff SP, Berg P (1976). „Construction of hybrid viruses containing SV40 and lambda phage DNA segments and their propagation in cultured monkey cells”. Cell. 9 (4 PT 2): 695—705. PMID 189942. doi:10.1016/0092-8674(76)90133-1. 

Literatura

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