TRNK pseudouridinska32 sintaza

TRNK pseudouridinska32 sintaza
Identifikatori
EC broj5.4.99.28
CAS broj430429-15-5
Baze podataka
IntEnzIntEnz pregled
BRENDABRENDA pristup
ExPASyNiceZyme pregled
KEGGKEGG pristup
MetaCycmetabolički put
PRIAMprofil
Strukture PBPRCSB PDB PDBe PDBj PDBsum

TRNK pseudouridinska32 sintaza (EC 5.4.99.28, RluA, pseudouridin sintaza RluA, Pus9p, Rib2/Pus8p) je enzim sa sistematskim imenom tRNK-uridin32 uracil mutaza.[1][2][3][4][5][6] Ovaj enzim katalizuje sledeću hemijsku reakciju

tRNK uridin32 tRNK pseudouridin32

Ovaj dualno specifični enzim iz Escherichia coli takođe katalizuje formiranje pseudouridina746 u molekulu 23S rRNK.

Reference

  1. ^ Hoang, C., Chen, J., Vizthum, C.A., Kandel, J.M., Hamilton, C.S., Mueller, E.G. and Ferre-D'Amare, A.R. (2006). „Crystal structure of pseudouridine synthase RluA: indirect sequence readout through protein-induced RNA structure”. Mol. Cell. 24: 535—545. PMID 17188032. 
  2. ^ Spedaliere, C.J., Hamilton, C.S. and Mueller, E.G. (2000). „Functional importance of motif I of pseudouridine synthases: mutagenesis of aligned lysine and proline residues”. Biochemistry. 39: 9459—9465. PMID 10924141. 
  3. ^ Raychaudhuri, S., Niu, L., Conrad, J., Lane, B.G. and Ofengand, J. (1999). „Functional effect of deletion and mutation of the Escherichia coli ribosomal RNA and tRNA pseudouridine synthase RluA”. J. Biol. Chem. 274: 18880—18886. PMID 10383384. 
  4. ^ Ramamurthy, V., Swann, S.L., Spedaliere, C.J. and Mueller, E.G. (1999). „Role of cysteine residues in pseudouridine synthases of different families”. Biochemistry. 38: 13106—13111. PMID 10529181. 
  5. ^ Wrzesinski, J., Nurse, K., Bakin, A., Lane, B.G. and Ofengand, J. (1995). „A dual-specificity pseudouridine synthase: an Escherichia coli synthase purified and cloned on the basis of its specificity for Ψ746 in 23S RNA is also specific for Ψ32 in tRNAPhe”. RNA. 1: 437—448. PMID 7493321. 
  6. ^ Behm-Ansmant, I., Grosjean, H., Massenet, S., Motorin, Y. and Branlant, C. (2004). „Pseudouridylation at position 32 of mitochondrial and cytoplasmic tRNAs requires two distinct enzymes in Saccharomyces cerevisiae”. J. Biol. Chem. 279: 52998—53006. PMID 15466869. 

Literatura

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