PROFILPELAJAR.COM

Pretraga
PMC	članci
PubMed	članci
NCBI	proteini

SARS koronavirusna glavna proteinaza
SARS koronavirusna glavna proteinaza
Identifikatori
EC broj	3.4.22.69
Baze podataka
IntEnz	IntEnz pregled
BRENDA	BRENDA pristup
ExPASy	NiceZyme pregled
KEGG	KEGG pristup
MetaCyc	metabolički put
PRIAM	profil
Strukture PBP	RCSB PDB PDBe PDBj PDBsum
PMC
Pretraga
PMC	članci
PubMed	članci
NCBI	proteini

SARS koronavirusna glavna proteinaza (EC 3.4.22.69, 3cLpro, 3C-slična proteaza, koronavirusna 3C-like proteaza, Mpro, SARS 3C-slična proteaza, SARS koronavirusna 3CL proteaza, SARS koronavirusna glavna peptidaza, SARS-CoV 3CLpro enzim, SARS-CoV glavna proteaza, SARS-CoV Mpro, glavna proteaza koronavirusnog jakog akutnog respiratornog sindroma) je enzim.^[1]^[2]^[3] Ovaj enzim katalizuje sledeću hemijsku reakciju

Najreaktivniji supstrati su peptidi TSAVLQ-SGFRK-NH₂ i SGVTFQ-GKFKK. Oni korespondiju mestima samo presecanja SARS 3C-sličnih proteinaza. Postoji preferencija za supstrate sa Gln u P1 poziciji i Leu u P2 poziciji

Glavna proteaza SARS koronavirusa je ključni enzim u obradi replikaznih poliproteina SARS koronavirusa.

Reference

^ Goetz, D.H., Choe, Y., Hansell, E., Chen, Y.T., McDowell, M., Jonsson, C.B., Roush, W.R., McKerrow, J. and Craik, C.S. (2007). „Substrate specificity profiling and identification of a new class of inhibitor for the major protease of the SARS coronavirus”. Biochemistry. 46: 8744—8752. PMID 17605471.
^ Fan, K., Wei, P., Feng, Q., Chen, S., Huang, C., Ma, L., Lai, B., Pei, J., Liu, Y., Chen, J. and Lai, L. (2004). „Biosynthesis, purification, and substrate specificity of severe acute respiratory syndrome coronavirus 3C-like proteinase”. J. Biol. Chem. 279: 1637—1642. PMID 14561748.
^ Akaji, K., Konno, H., Onozuka, M., Makino, A., Saito, H. and Nosaka, K. (2008). „Evaluation of peptide-aldehyde inhibitors using R188I mutant of SARS 3CL protease as a proteolysis-resistant mutant”. Bioorg. Med. Chem. 16: 9400—9408. PMID 18845442.

Literatura

Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
William P. Jencks (1987). Catalysis in Chemistry and Enzymology. Dover Publications. ISBN 0486654605.

Spoljašnje veze

SARS+coronavirus+main+proteinase на US National Library of Medicine Medical Subject Headings (MeSH)

[1] Goetz, D.H., Choe, Y., Hansell, E., Chen, Y.T., McDowell, M., Jonsson, C.B., Roush, W.R., McKerrow, J. and Craik, C.S. (2007). „Substrate specificity profiling and identification of a new class of inhibitor for the major protease of the SARS coronavirus”. Biochemistry. 46: 8744—8752. PMID 17605471.

[2] Fan, K., Wei, P., Feng, Q., Chen, S., Huang, C., Ma, L., Lai, B., Pei, J., Liu, Y., Chen, J. and Lai, L. (2004). „Biosynthesis, purification, and substrate specificity of severe acute respiratory syndrome coronavirus 3C-like proteinase”. J. Biol. Chem. 279: 1637—1642. PMID 14561748.

[3] Akaji, K., Konno, H., Onozuka, M., Makino, A., Saito, H. and Nosaka, K. (2008). „Evaluation of peptide-aldehyde inhibitors using R188I mutant of SARS 3CL protease as a proteolysis-resistant mutant”. Bioorg. Med. Chem. 16: 9400—9408. PMID 18845442.

[1]

[2]

[3]