Metanol dehidrogenaza (nikotinoprotein)

Metanol dehidrogenaza (nikotinoprotein)
Identifikatori
EC broj1.1.99.37
Baze podataka
IntEnzIntEnz pregled
BRENDABRENDA pristup
ExPASyNiceZyme pregled
KEGGKEGG pristup
MetaCycmetabolički put
PRIAMprofil
Strukture PBPRCSB PDB PDBe PDBj PDBsum

Metanol dehidrogenaza (nikotinoprotein) (EC 1.1.99.37, NDMA-zavisna metanolna dehidrogenaza, nikotinoprotein metanolna dehidrogenaza, metanol:N,N-dimetil-4-nitrosoaniline oksidoreduktaza) je enzim sa sistematskim imenom metanol:akceptor oksidoreduktaza.[1][2][3][4][5] Ovaj enzim katalizuje sledeću hemijsku reakciju

metanol + akceptor formaldehid + redukovani akceptor

Ovaj enzim sadrži Zn2+ i Mg2+. Nikotinoprotein metanolne dehidrogenaze sadrže čvrsto vezani NADP+/NADPH kofaktor koji se ne disocira tokom katalitičkog procesa. Umesto toga kofaktor se regeneratiše drugim supstratom ili nosiocem elektrona.

Reference

  1. ^ Vonck, J., Arfman, N., De Vries, G.E., Van Beeumen, J., Van Bruggen, E.F. and Dijkhuizen, L. (1991). „Electron microscopic analysis and biochemical characterization of a novel methanol dehydrogenase from the thermotolerant Bacillus sp. C1”. J. Biol. Chem. 266: 3949—3954. PMID 1995642. 
  2. ^ Van Ophem, P.W., Van Beeumen, J. and Duine, J.A. (1993). „Nicotinoprotein [NAD(P)-containing] alcohol/aldehyde oxidoreductases. Purification and characterization of a novel type from Amycolatopsis methanolica”. Eur. J. Biochem. 212: 819—826. PMID 8385013. 
  3. ^ Bystrykh, L.V., Vonck, J., van Bruggen, E.F., van Beeumen, J., Samyn, B., Govorukhina, N.I., Arfman, N., Duine, J.A. and Dijkhuizen, L. (1993). „Electron microscopic analysis and structural characterization of novel NADP(H)-containing methanol: N,N′-dimethyl-4-nitrosoaniline oxidoreductases from the gram-positive methylotrophic bacteria Amycolatopsis methanolica and Mycobacterium gastri MB19”. J. Bacteriol. 175: 1814—1822. PMID 8449887. 
  4. ^ Hektor, H.J., Kloosterman, H. and Dijkhuizen, L. (2002). „Identification of a magnesium-dependent NAD(P)(H)-binding domain in the nicotinoprotein methanol dehydrogenase from Bacillus methanolicus”. J. Biol. Chem. 277: 46966—46973. PMID 12351635. 
  5. ^ Park, H., Lee, H., Ro, Y.T. and Kim, Y.M. (2010). „Identification and functional characterization of a gene for the methanol : N,N′-dimethyl-4-nitrosoaniline oxidoreductase from Mycobacterium sp. strain JC1 (DSM 3803)”. Microbiology. 156: 463—471. PMID 19875438. 

Literatura

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