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Pretraga
PMC	članci
PubMed	članci
NCBI	proteini

Kostunolid sintaza
Kostunolid sintaza
Identifikatori
EC broj	1.14.13.120
Baze podataka
IntEnz	IntEnz pregled
BRENDA	BRENDA pristup
ExPASy	NiceZyme pregled
KEGG	KEGG pristup
MetaCyc	metabolički put
PRIAM	profil
Strukture PBP	RCSB PDB PDBe PDBj PDBsum
PMC
Pretraga
PMC	članci
PubMed	članci
NCBI	proteini

Kostunolid sintaza (EC 1.14.13.120, kostunolidna sintaza) je enzim sa sistematskim imenom germakra-1(10),4,11(13)-trien-12-oat,NADPH:kiseonik oksidoreduktaza (6alfa-hidroksilacija).^[1] Ovaj enzim katalizuje sledeću hemijsku reakciju

germakra-1(10),4,11(13)-trien-12-oat + NADPH + H⁺ + O₂

\rightleftharpoons

(+)-kostunolid + NADP⁺ + 2H₂O

A heme-tiolate protein (P-450). Ovaj enzim hidroksiliše ugljenik C-6 germakra-1(10),4,11(13)-trien-12-oata i daje 6alfa-hidroksigermakra-1(10),4,11(13)-trien-12-oat, koji se spontano ciklizuje i formira laktonski prsten.

Reference

^ de Kraker, J.W., Franssen, M.C., Joerink, M., de Groot, A. and Bouwmeester, H.J. (2002). „Biosynthesis of costunolide, dihydrocostunolide, and leucodin. Demonstration of cytochrome p₄₅₀-catalyzed formation of the lactone ring present in sesquiterpene lactones of chicory”. Plant Physiol. 129: 257—268. PMID 12011356.

Literatura

Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
William P. Jencks (1987). Catalysis in Chemistry and Enzymology. Dover Publications. ISBN 0486654605.

Spoljašnje veze

Costunolide+synthase на US National Library of Medicine Medical Subject Headings (MeSH)

[1] Kraker, J.W., Franssen, M.C., Joerink, M., de Groot, A. and Bouwmeester, H.J. (2002). „Biosynthesis of costunolide, dihydrocostunolide, and leucodin. Demonstration of cytochrome p₄₅₀-catalyzed formation of the lactone ring present in sesquiterpene lactones of chicory”. Plant Physiol. 129: 257—268. PMID 12011356.

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