Kapa-karaginaza

Kapa-karaginaza
Identifikatori
EC broj3.2.1.83
CAS broj37288-59-8
Baze podataka
IntEnzIntEnz pregled
BRENDABRENDA pristup
ExPASyNiceZyme pregled
KEGGKEGG pristup
MetaCycmetabolički put
PRIAMprofil
Strukture PBPRCSB PDB PDBe PDBj PDBsum

Kapa-karaginaza (EC 3.2.1.83, kapa-karaginan 4-beta-D-glikanohidrolaza) je enzim sa sistematskim imenom kapa-karaginan 4-beta-D-glikanohidrolaza (zadržavanje konfiguracije).[1][2][3][4][5] Ovaj enzim katalizuje sledeću hemijsku reakciju

Endohidroliza (1->4)-beta-D-veza između D-galaktoza 4-sulfata i 3,6-anhidro-D-galaktoze u kapa-karagenanima

Glavni produkti hidrolize su neokarabioza-sulfat i neokaratetraoza-sulfat.

Reference

  1. ^ Weigl, J. & Yashe, W. (1966). „The enzymic hydrolysis of carrageenan by Pseudomonas carrageenovora: purification of a κ-carrageenase”. Can. J. Microbiol. 12: 939—947. PMID 5972647. 
  2. ^ Potin, P., Sanseau, A., Le Gall, Y., Rochas, C. and Kloareg, B. (1991). „Purification and characterization of a new κ-carrageenase from a marine Cytophaga-like bacterium”. Eur. J. Biochem. 201: 241—247. PMID 1915370. 
  3. ^ Potin, P., Richard, C., Barbeyron, T., Henrissat, B., Gey, C., Petillot, Y., Forest, E., Dideberg, O., Rochas, C. and Kloareg, B. (1995). „Processing and hydrolytic mechanism of the cgkA-encoded κ-carrageenase of Alteromonas carrageenovora”. Eur. J. Biochem. 228: 971—975. PMID 7737202. 
  4. ^ Michel, G., Barbeyron, T., Flament, D., Vernet, T., Kloareg, B. and Dideberg, O. (1999). „Expression, purification, crystallization and preliminary x-ray analysis of the κ-carrageenase from Pseudoalteromonas carrageenovora”. Acta Crystallogr. D Biol. Crystallogr. 55: 918—920. PMID 10089334. 
  5. ^ Michel, G., Chantalat, L., Duee, E., Barbeyron, T., Henrissat, B., Kloareg, B. and Dideberg, O. (2001). „The κ-carrageenase of P. carrageenovora features a tunnel-shaped active site: a novel insight in the evolution of Clan-B glycoside hydrolases”. Structure. 9: 513—525. PMID 11435116. 

Literatura

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