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Flavonoid 3',5'-hidroksilaza
Flavonoid 3',5'-hidroksilaza
Identifikatori
EC broj	1.14.13.88
CAS broj	94047-23-1
Baze podataka
IntEnz	IntEnz pregled
BRENDA	BRENDA pristup
ExPASy	NiceZyme pregled
KEGG	KEGG pristup
MetaCyc	metabolički put
PRIAM	profil
Strukture PBP	RCSB PDB PDBe PDBj PDBsum
PMC
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PMC	članci
PubMed	članci
NCBI	proteini

Flavonoid 3',5'-hidroksilaza (EC 1.14.13.88, flavonoidna 3',5'-hidroksilaza) je enzim sa sistematskim imenom flavanon,NADPH:kiseonik oksidoreduktaza.^[1]^[2]^[3] Ovaj enzim katalizuje sledeću hemijsku reakciju

flavanon + 2 NADPH + 2 H⁺ + 2 O₂

\rightleftharpoons

3',5'-dihidroksiflavanon + 2 NADP⁺ + 2H₂O (sveukupna reakcija)

(1a) flavanon + NADPH + H⁺ + O₂

\rightleftharpoons

3'-hidroksiflavanon + NADP⁺ + H₂O

(1b) 3'-hidroksiflavanon + NADPH + H⁺ + O₂

\rightleftharpoons

3',5'-dihidroksiflavanon + NADP⁺ + H₂O

Ovaj enzim je hem-tiolatni protein (P-450). 3',5'-dihidroksiflavanon se formira putem 3'-hidroksiflavanona.

Reference

^ Menting, J., Scopes, R.K. and Stevenson, T.W. (1994). „Characterization of flavonoid 3′,5′-hydroxylase in microsomal membrane fraction of Petunia hybrida flowers”. Plant Physiol. 106: 633—642. PMID 12232356.
^ Shimada, Y., Nakano-Shimada, R., Ohbayashi, M., Okinaka, Y., Kiyokawa, S. and Kikuchi, Y. (1999). „Expression of chimeric P450 genes encoding flavonoid-3′, 5′-hydroxylase in transgenic tobacco and petunia plants¹”. FEBS Lett. 461: 241—245. PMID 10567704.
^ de Vetten, N., ter Horst, J., van Schaik, H.P., de Boer, A., Mol, J. and Koes, R. (1999). „A cytochrome b₅ is required for full activity of flavonoid 3′, 5′-hydroxylase, a cytochrome P450 involved in the formation of blue flower colors”. Proc. Natl. Acad. Sci. USA. 96: 778—783. PMID 9892710.

Literatura

Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
William P. Jencks (1987). Catalysis in Chemistry and Enzymology. Dover Publications. ISBN 0486654605.

Spoljašnje veze

Flavanoid+3',5'-hydroxylase на US National Library of Medicine Medical Subject Headings (MeSH)

[1] Menting, J., Scopes, R.K. and Stevenson, T.W. (1994). „Characterization of flavonoid 3′,5′-hydroxylase in microsomal membrane fraction of Petunia hybrida flowers”. Plant Physiol. 106: 633—642. PMID 12232356.

[2] Shimada, Y., Nakano-Shimada, R., Ohbayashi, M., Okinaka, Y., Kiyokawa, S. and Kikuchi, Y. (1999). „Expression of chimeric P450 genes encoding flavonoid-3′, 5′-hydroxylase in transgenic tobacco and petunia plants¹”. FEBS Lett. 461: 241—245. PMID 10567704.

[3] Vetten, N., ter Horst, J., van Schaik, H.P., de Boer, A., Mol, J. and Koes, R. (1999). „A cytochrome b₅ is required for full activity of flavonoid 3′, 5′-hydroxylase, a cytochrome P450 involved in the formation of blue flower colors”. Proc. Natl. Acad. Sci. USA. 96: 778—783. PMID 9892710.

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