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Pretraga
PMC	članci
PubMed	članci
NCBI	proteini

Ent-kauren oksidaza
Ent-kauren oksidaza
Identifikatori
EC broj	1.14.13.78
CAS broj	149565-67-3
Baze podataka
IntEnz	IntEnz pregled
BRENDA	BRENDA pristup
ExPASy	NiceZyme pregled
KEGG	KEGG pristup
MetaCyc	metabolički put
PRIAM	profil
Strukture PBP	RCSB PDB PDBe PDBj PDBsum
PMC
Pretraga
PMC	članci
PubMed	članci
NCBI	proteini

Ent-kauren oksidaza (EC 1.14.13.78, ent-kaurenska oksidaza) je enzim sa sistematskim imenom ent-kaur-16-en,NADPH:kiseonik oksidoreduktaza (hidroksilacija).^[1]^[2]^[3] Ovaj enzim katalizuje sledeću hemijsku reakciju

ent-kaur-16-en + 3 NADPH + 3 H⁺ + 3 O₂

\rightleftharpoons

ent-kaur-16-en-19-oat + 3 NADP⁺ + 4H₂O (sveukupna reakcija)

(1a) ent-kaur-16-en + NADPH + H⁺ + O₂

\rightleftharpoons

ent-kaur-16-en-19-ol + NADP⁺ + H₂O

(1b) ent-kaur-16-en-19-ol + NADPH + H⁺ + O₂

\rightleftharpoons

ent-kaur-16-en-19-al + NADP⁺ + 2H₂O

(1c) ent-kaur-16-en-19-al + NADPH + O₂

\rightleftharpoons

ent-kaur-16-en-19-oat + NADP⁺ + H₂O

Za rad ovog enzima je neophodan citohrom P450. On katalizuje tri sukcesivne oksidacije 4-metil grupe ent-kaurena dajući kaurenoinsku kiselinu.

Reference

^ Ashman, P.J., Mackenzie, A. and Bramley, P.M. (1990). „Characterization of ent-kaurene oxidase activity from Gibberella fujikuroi”. Biochim. Biophys. Acta. 1036: 151—157. PMID 2223832.
^ Archer, C., Ashman, P.J., Hedden, P., Bowyer, J.R. and Bramley, P.M. (1992). „Purification of ent-kaurene oxidase from Gibberella fujikuroi and Cucurbita maxima”. Biochem. Soc. Trans. 20: 218—218. PMID 1397591.
^ Helliwell, C.A., Poole, A., Peacock, W.J. and Dennis, E.S. (1999). „Arabidopsis ent-kaurene oxidase catalyzes three steps of gibberellin biosynthesis”. Plant Physiol. 119: 507—510. PMID 9952446.

Literatura

Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
William P. Jencks (1987). Catalysis in Chemistry and Enzymology. Dover Publications. ISBN 0486654605.

Spoljašnje veze

Ent-kaurene+oxidase на US National Library of Medicine Medical Subject Headings (MeSH)

[1] Ashman, P.J., Mackenzie, A. and Bramley, P.M. (1990). „Characterization of ent-kaurene oxidase activity from Gibberella fujikuroi”. Biochim. Biophys. Acta. 1036: 151—157. PMID 2223832.

[2] Archer, C., Ashman, P.J., Hedden, P., Bowyer, J.R. and Bramley, P.M. (1992). „Purification of ent-kaurene oxidase from Gibberella fujikuroi and Cucurbita maxima”. Biochem. Soc. Trans. 20: 218—218. PMID 1397591.

[3] Helliwell, C.A., Poole, A., Peacock, W.J. and Dennis, E.S. (1999). „Arabidopsis ent-kaurene oxidase catalyzes three steps of gibberellin biosynthesis”. Plant Physiol. 119: 507—510. PMID 9952446.

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