CMP-N-acetilneuraminat monooksigenaza

CMP-N-acetilneuraminat monooksigenaza
Identifikatori
EC broj1.14.18.2
CAS broj116036-67-0
Baze podataka
IntEnzIntEnz pregled
BRENDABRENDA pristup
ExPASyNiceZyme pregled
KEGGKEGG pristup
MetaCycmetabolički put
PRIAMprofil
Strukture PBPRCSB PDB PDBe PDBj PDBsum

CMP-N-acetilneuraminat monooksigenaza (EC 1.14.18.2, CMP-N-acetilneuraminska kiselina hidroksilaza, CMP-Neu5Ac hidroksilaza, citidin monofosfoacetilneuraminatna monooksigenaza, N-acetilneuraminska monooksigenaza, citidin-5'-monofosfat-N-acetilneuraminska kiselina hidroksilaza) je enzim sa sistematskim imenom CMP-N-acetilneuraminat,ferocitohrom-b5:kiseonik oksidoreduktaza (N-acetil-hidroksilacija).[1][2][3][4][5] Ovaj enzim katalizuje sledeću hemijsku reakciju

CMP-N-acetilneuraminat + 2 ferocitohrom b5 + O2 + 2 H+ CMP-N-glikoloilneuraminat + 2 fericitohrom b5 + H2O

Ovaj enzim sadrži Riskijev tip [2Fe-2S] klastera i jedan dodatno mesto vezivanja gvožđa.

Reference

  1. ^ Shaw, L. & Schauer, R. (1988). „The biosynthesis of N-glycoloylneuraminic acid occurs by hydroxylation of the CMP-glycoside of N-acetylneuraminic acid”. Biol. Chem. Hoppe-Seyler. 369: 477—486. PMID 3202954. 
  2. ^ Kozutsumi, Y., Kawano, T., Yamakawa, T. and Suzuki, A. (1990). „Participation of cytochrome b5 in CMP-N-acetylneuraminic acid hydroxylation in mouse liver cytosol”. J. Biochem. (Tokyo). 109: 704—706. PMID 1964451. 
  3. ^ Schneckenburger, P., Shaw, L. and Schauer, R. (1994). „Purification, characterization and reconstitution of CMP-N-acetylneuraminate hydroxylase from mouse liver”. Glycoconj. J. 11: 194—203. PMID 7841794. 
  4. ^ Kawano, T., Koyama, S., Takematsu, H., Kozutsumi, Y., Kawasaki, H., Kawashima, S., Kawasaki, T. and Suzuki, A. (1995). „Molecular cloning of cytidine monophospho-N-acetylneuraminic acid hydroxylase. Regulation of species- and tissue-specific expression of N-glycolylneuraminic acid”. J. Biol. Chem. 270: 16458—16463. PMID 7608218. 
  5. ^ Schlenzka, W., Shaw, L., Kelm, S., Schmidt, C.L., Bill, E., Trautwein, A.X., Lottspeich, F. and Schauer, R. (1996). „CMP-N-acetylneuraminic acid hydroxylase: the first cytosolic Rieske iron-sulphur protein to be described in Eukarya”. FEBS Lett. 385: 197—200. PMID 8647250. 

Literatura

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