Biotin sintaza

Biotin sintaza
Identifikatori
EC broj2.8.1.6
CAS broj80146-93-6
Baze podataka
IntEnzIntEnz pregled
BRENDABRENDA pristup
ExPASyNiceZyme pregled
KEGGKEGG pristup
MetaCycmetabolički put
PRIAMprofil
Strukture PBPRCSB PDB PDBe PDBj PDBsum

Biotin sintaza (EC 2.8.1.6, Biotin synthase) je enzim sa sistematskim imenom detiobiotin:sumpor sumportransferaza.[1][2][3][4][5][6][7][8] Ovaj enzim katalizuje sledeću hemijsku reakciju

detiobiotin + [S] + 2 S-adenozil-L-metionin biotin + 2 L-metionin + 2 5'-dezoksiadenozin

Ovaj enzim vezuje [4Fe-4S] i [2Fe-2S] klaster.

Reference

  1. ^ Trainor, D.A., Parry, R.J. and Gitterman, A. (1980). „Biotin biosynthesis. 2. Stereochemistry of sulfur introduction at C-4 of dethiobiotin”. J. Am. Chem. Soc. 102: 1467—1468. 
  2. ^ Shiuan, D. & Campbell, A. (1988). „Transcriptional regulation and gene arrangement of Escherichia coli, Citrobacter freundii and Salmonella typhimurium biotin operons”. Gene. 67: 203—211. PMID 2971595. 
  3. ^ Zhang, S., Sanyal, I., Bulboaca, G.H., Rich, A. and Flint, D.H. (1994). „The gene for biotin synthase from Saccharomyces cerevisiae: cloning, sequencing, and complementation of Escherichia coli strains lacking biotin synthase”. Arch. Biochem. Biophys. 309: 29—35. PMID 8117110. 
  4. ^ Ugulava, N.B., Gibney, B.R. and Jarrett, J.T. (2001). „Biotin synthase contains two distinct iron-sulfur cluster binding sites: chemical and spectroelectrochemical analysis of iron-sulfur cluster interconversions”. Biochemistry. 40: 8343—8351. PMID 11444981. 
  5. ^ Berkovitch, F., Nicolet, Y., Wan, J.T., Jarrett, J.T. and Drennan, C.L. (2004). „Crystal structure of biotin synthase, an S-adenosylmethionine-dependent radical enzyme”. Science. 303: 76—79. PMID 14704425. 
  6. ^ Lotierzo, M., Tse Sum Bui, B., Florentin, D., Escalettes, F. and Marquet, A. (2005). „Biotin synthase mechanism: an overview”. Biochem. Soc. Trans. 33: 820—823. PMID 16042606. 
  7. ^ Taylor, A.M., Farrar, C.E. and Jarrett, J.T. (2008). „9-Mercaptodethiobiotin is formed as a competent catalytic intermediate by Escherichia coli biotin synthase”. Biochemistry. 47: 9309—9317. PMID 18690713. 
  8. ^ Reyda, M.R., Fugate, C.J. and Jarrett, J.T. (2009). „A complex between biotin synthase and the iron-sulfur cluster assembly chaperone HscA that enhances in vivo cluster assembly”. Biochemistry. 48: 10782—10792. PMID 19821612. 

Literatura

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