Alifatična (R)-hidroksinitrilna lijaza

Alifatična (R)-hidroksinitrilna lijaza
Identifikatori
EC broj4.1.2.46
Baze podataka
IntEnzIntEnz pregled
BRENDABRENDA pristup
ExPASyNiceZyme pregled
KEGGKEGG pristup
MetaCycmetabolički put
PRIAMprofil
Strukture PBPRCSB PDB PDBe PDBj PDBsum

Alifatična (R)-hidroksinitrilna lijaza (EC 4.1.2.46, (R)-HNL, (R)-oksinitrilaza, (R)-hidroksinitril lijaza, LuHNL) je enzim sa sistematskim imenom (2R)-2-hidroksi-2-metilbutannitril butan-2-on-lijaza (formira cijanid).[1][2][3][4][5][6] Ovaj enzim katalizuje sledeću hemijsku reakciju

(2R)-2-hidroksi-2-metilbutannitril cijanid + butan-2-on

Ovaj enzim sadrži Zn2+.

Reference

  1. ^ Trummler, K., Roos, J., Schwaneberg, U., Effenberger, F., Förster, S., Pfizenmaier, K. and Wajant, H. (1998). „Expression of the Zn2+-containing hydroxynitrile lyase from flax (Linum usitatissimum) in Pichia pastoris– utilization of the recombinant enzyme for enzymatic analysis and site-directed mutagenesis”. Plant Sci. 139: 19—27. 
  2. ^ Trummler, K. & Wajant, H. (1997). „Molecular cloning of acetone cyanohydrin lyase from flax (Linum usitatissimum). Definition of a novel class of hydroxynitrile lyases”. J. Biol. Chem. 272: 4770—4774. PMID 9030531. 
  3. ^ Albrecht, J., Jansen, I.and Kula, M.R. (1993). „Improved purification of an (R)-oxynitrilase from Linum usitatissimum (flax) and investigation of the substrate range”. Biotechnol. Appl. Biochem. 17: 191—203. 
  4. ^ Xu, L.-L., Singh, B.K. and Conn, E.E. (1988). „Purification and characterization of acetone cyanohydrin lyase from Linum usitatissimum”. Arch. Biochem. Biophys. 263: 256—263. PMID 3377504. 
  5. ^ Cabirol, F.L., Tan, P.L., Tay, B., Cheng, S., Hanefeld, U. and Sheldon, R.A. (2008). „Linum usitatissimum hydroxynitrile lyase cross-linked enzyme aggregates: a recyclable enantioselective catalyst”. Adv. Synth. Catal. 350: 2329—2338. 
  6. ^ Breithaupt, H., Pohl, M., Bönigk, W., Heim, P., Schimz, K.-L. and Kula, M.-R. (1999). „Cloning and expression of (R)-hydroxynitrile lyase from Linum usitatissimum (flax)”. J. Mol. Catal. B. 6: 315—332. 

Literatura

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