Acil-homoserin-lakton sintaza

Acil-homoserin-lakton sintaza
Identifikatori
EC broj2.3.1.184
CAS broj176023-66-8
Baze podataka
IntEnzIntEnz pregled
BRENDABRENDA pristup
ExPASyNiceZyme pregled
KEGGKEGG pristup
MetaCycmetabolički put
PRIAMprofil
Strukture PBPRCSB PDB PDBe PDBj PDBsum

Acil-homoserin-lakton sintaza (EC 2.3.1.184, acil-homoserin laktonska sintaza, acil homoserin laktonska sintaza, acil-homoserinlakton sintaza, acilhomoserin laktonska sintaza, AHL sintaza, AHS, AHSL sintaza, AhyI, AinS, AinS protein, autoinducer sintaza, autoinducer sinteza protein rhlI, EsaI, ExpISCC1, ExpISCC3065, LasI, LasR, LuxI, LuxI protein, LuxM, N-acil homoserin lakton sintaza, RhlI, YspI, acil-(acil nosilac protein):S-adenozil-L-metionin aciltranseraza (formira lakton, otpušta metiltioadenozin)) je enzim sa sistematskim imenom acil-(acil-nosilac protein):S-adenozil-L-metionin aciltranseraza (formira lakton).[1][2][3][4][5][6][7][8][9] Ovaj enzim katalizuje sledeću hemijsku reakciju

acil-[acil-nosilac protein] + S-adenozil-L-metionin [acil-nosilac protein] + S-metil-5'-tioadenozin + N-acil-L-homoserin lakton

Acil-homoserinske laktone formiraju brojne bakterijske vrste. Oni se koriste za regulaciju izražavanja gena virulencije u procesu poznatom kao očitavanje kvoruma.

Reference

  1. ^ Schaefer, A.L., Val, D.L., Hanzelka, B.L., Cronan, J.E., Jr. and Greenberg, E.P. (1996). „Generation of cell-to-cell signals in quorum sensing: acyl homoserine lactone synthase activity of a purified Vibrio fischeri LuxI protein”. Proc. Natl. Acad. Sci. USA. 93: 9505—9509. PMID 8790360. 
  2. ^ Watson, W.T., Murphy, F.V., 4th, Gould, T.A., Jambeck, P., Val, D.L., Cronan, J.E., Jr., Beck von Bodman, S. and Churchill, M.E. (2001). „Crystallization and rhenium MAD phasing of the acyl-homoserinelactone synthase EsaI”. Acta Crystallogr. D Biol. Crystallogr. 57: 1945—1949. PMID 11717525. 
  3. ^ Chakrabarti, S. & Sowdhamini, R. (2003). „Functional sites and evolutionary connections of acylhomoserine lactone synthases”. Protein Eng. 16: 271—278. PMID 12736370. 
  4. ^ Hanzelka, B.L., Parsek, M.R., Val, D.L., Dunlap, P.V., Cronan, J.E., Jr. and Greenberg, E.P. (1999). „Acylhomoserine lactone synthase activity of the Vibrio fischeri AinS protein”. J. Bacteriol. 181: 5766—5770. PMID 10482519. 
  5. ^ Parsek, M.R., Val, D.L., Hanzelka, B.L., Cronan, J.E., Jr. and Greenberg, E.P. (1999). „Acyl homoserine-lactone quorum-sensing signal generation”. Proc. Natl. Acad. Sci. USA. 96: 4360—4365. PMID 10200267. 
  6. ^ Ulrich, R.L. (2004). „Quorum quenching: enzymatic disruption of N-acylhomoserine lactone-mediated bacterial communication in Burkholderia thailandensis”. Appl. Environ. Microbiol. 70: 6173—6180. PMID 15466564. 
  7. ^ Gould, T.A., Schweizer, H.P. and Churchill, M.E. (2004). „Structure of the Pseudomonas aeruginosa acyl-homoserinelactone synthase LasI”. Mol. Microbiol. 53: 1135—1146. PMID 15306017. 
  8. ^ Raychaudhuri, A., Jerga, A. and Tipton, P.A. (2005). „Chemical mechanism and substrate specificity of RhlI, an acylhomoserine lactone synthase from Pseudomonas aeruginosa”. Biochemistry. 44: 2974—2981. PMID 15723540. 
  9. ^ Gould, T.A., Herman, J., Krank, J., Murphy, R.C. and Churchill, M.E. (2006). „Specificity of acyl-homoserine lactone synthases examined by mass spectrometry”. J. Bacteriol. 188: 773—783. PMID 16385066. 

Literatura

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