TRNK pseudouridinska32 sintaza

TRNK pseudouridinska32 sintaza
Identifikatori
EC broj 5.4.99.28
CAS broj 430429-15-5
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB RCSB PDB PDBe PDBj PDBsum

TRNK pseudouridinska32 sintaza (EC 5.4.99.28, RluA, pseudouridin sintaza RluA, Pus9p, Rib2/Pus8p) je enzim sa sistematskim imenom tRNK-uridin32 uracil mutaza.[1][2][3][4][5][6] Ovaj enzim katalizuje sledeću hemijsku reakciju

tRNK uridin32 tRNK pseudouridin32

Ovaj dualno specifični enzim iz Escherichia coli takođe katalizuje formiranje pseudouridina746 u molekulu 23S rRNK.

Reference

  1. ↑ Hoang, C., Chen, J., Vizthum, C.A., Kandel, J.M., Hamilton, C.S., Mueller, E.G. and Ferre-D'Amare, A.R. (2006). „Crystal structure of pseudouridine synthase RluA: indirect sequence readout through protein-induced RNA structure”. Mol. Cell 24: 535-545. PMID 17188032. 
  2. ↑ Spedaliere, C.J., Hamilton, C.S. and Mueller, E.G. (2000). „Functional importance of motif I of pseudouridine synthases: mutagenesis of aligned lysine and proline residues”. Biochemistry 39: 9459-9465. PMID 10924141. 
  3. ↑ Raychaudhuri, S., Niu, L., Conrad, J., Lane, B.G. and Ofengand, J. (1999). „Functional effect of deletion and mutation of the Escherichia coli ribosomal RNA and tRNA pseudouridine synthase RluA”. J. Biol. Chem. 274: 18880-18886. PMID 10383384. 
  4. ↑ Ramamurthy, V., Swann, S.L., Spedaliere, C.J. and Mueller, E.G. (1999). „Role of cysteine residues in pseudouridine synthases of different families”. Biochemistry 38: 13106-13111. PMID 10529181. 
  5. ↑ Wrzesinski, J., Nurse, K., Bakin, A., Lane, B.G. and Ofengand, J. (1995). „A dual-specificity pseudouridine synthase: an Escherichia coli synthase purified and cloned on the basis of its specificity for Ψ746 in 23S RNA is also specific for Ψ32 in tRNAPhe”. RNA 1: 437-448. PMID 7493321. 
  6. ↑ Behm-Ansmant, I., Grosjean, H., Massenet, S., Motorin, Y. and Branlant, C. (2004). „Pseudouridylation at position 32 of mitochondrial and cytoplasmic tRNAs requires two distinct enzymes in Saccharomyces cerevisiae”. J. Biol. Chem. 279: 52998-53006. PMID 15466869. 

Literatura

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