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Retinol dehidrogenaza
Identifikatori
EC broj	1.1.1.105
CAS broj	9033-53-8
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB	RCSB PDB PDBe PDBj PDBsum
Pretraga
PMC	articles
PubMed	articles
NCBI Protein	search

Retinol dehidrogenaza (EC 1.1.1.105, retinol (vitamin A1) dehidrogenaza, MDR, mikrozomalna retinolna dehidrogenaza, retinalna reduktaza, retinenska reduktaza, epidermalna retinol dehidrogenaza 2, SDR16C5, RDH16) je enzim sa sistematskim imenom all-trans retinol:NAD⁺ oksidoreduktaza.^[1]^[2]^[3]^[4] Ovaj enzim katalizuje sledeću hemijsku reakciju

sve-trans-retinol---[ćelijski protein vezivanja retinola] + NAD⁺

\rightleftharpoons

sve-trans-retinal---[ćelijski protein vezivanja retinola] + NADH + H⁺

Retinolna dehidrogenaza prepoznaje sve-trans-retinol i sve-trans-retinal kao supstrate i ispoljava jaku preferenciju za NAD⁺/NADH kao kofaktore. Ona prepoznaje supstrat u slobodnoj formi, kao i kad je vezan za ćelijski-retinol-vezujući-protein (CRBP1), ali ima veći afinitet za supstrat u vezanoj formi. Retinolna dehidrogenaza nije aktivna na 11-cis-retinolu ili 11-cis-retinala (cf. EC 1.1.1.315, 11-cis retinol dehidrogenaza). Ona je deluje na 3alfa-hidroksisteroide.

Reference

↑ Koen, A.L. and Shaw, C.R. (1966). „Retinol and alcohol dehydrogenases in retina and liver”. Biochim. Biophys. Acta 128: 48-54. PMID 5972368.
↑ Gough, W.H., VanOoteghem, S., Sint, T. and Kedishvili, N.Y. (1998). „cDNA cloning and characterization of a new human microsomal NAD⁺-dependent dehydrogenase that oxidizes all-trans-retinol and 3α-hydroxysteroids”. J. Biol. Chem. 273: 19778-19785. PMID 9677409.
↑ Matsuzaka, Y., Okamoto, K., Tsuji, H., Mabuchi, T., Ozawa, A., Tamiya, G. and Inoko, H. (2002). „Identification of the hRDH-E2 gene, a novel member of the SDR family, and its increased expression in psoriatic lesion”. Biochem. Biophys. Res. Commun. 297: 1171-1180. PMID 12372410.
↑ Lee, S.A., Belyaeva, O.V. and Kedishvili, N.Y. (2009). „Biochemical characterization of human epidermal retinol dehydrogenase 2”. Chem. Biol. Interact. 178: 182-187. PMID 18926804.

Literatura

Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.

Vanjske veze

MeSH All-trans-retinol+dehydrogenase+(NAD+)

[1] Koen, A.L. and Shaw, C.R. (1966). „Retinol and alcohol dehydrogenases in retina and liver”. Biochim. Biophys. Acta 128: 48-54. PMID 5972368.

[2] Gough, W.H., VanOoteghem, S., Sint, T. and Kedishvili, N.Y. (1998). „cDNA cloning and characterization of a new human microsomal NAD⁺-dependent dehydrogenase that oxidizes all-trans-retinol and 3α-hydroxysteroids”. J. Biol. Chem. 273: 19778-19785. PMID 9677409.

[3] Matsuzaka, Y., Okamoto, K., Tsuji, H., Mabuchi, T., Ozawa, A., Tamiya, G. and Inoko, H. (2002). „Identification of the hRDH-E2 gene, a novel member of the SDR family, and its increased expression in psoriatic lesion”. Biochem. Biophys. Res. Commun. 297: 1171-1180. PMID 12372410.

[4] Lee, S.A., Belyaeva, O.V. and Kedishvili, N.Y. (2009). „Biochemical characterization of human epidermal retinol dehydrogenase 2”. Chem. Biol. Interact. 178: 182-187. PMID 18926804.

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