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Ramnogalakturonanska hidrolaza
Identifikatori
EC broj	3.2.1.171
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB	RCSB PDB PDBe PDBj PDBsum
Pretraga
PMC	articles
PubMed	articles
NCBI Protein	search

Ramnogalakturonanska hidrolaza (EC 3.2.1.171, ramnogalakturonaza A, RGaza A, RG-hidrolaza) je enzim sa sistematskim imenom ramnogalakturonan alfa-D-GalA-(1->2)-alfa-L-Rha hidrolaza.^[1]^[2]^[3]^[4]^[5] Ovaj enzim katalizuje sledeću hemijsku reakciju

Endohidroliza alfa-D-GalA-(1->2)-alfa-L-Rha glikozidne veze u ramnogalakturonan I osnovi sa inicijalnom inverzijom anomerne konfiguracije čime se odvajaju oligosaharidi sa beta-D-GalA ostatkom na redukujućem kraju

Ovaj enzim učestvuje u degradaciji ramnogalakturonana I kod Aspergillus aculeatus.

Reference

↑ Petersen, T.N., Kauppinen, S. and Larsen, S. (1997). „The crystal structure of rhamnogalacturonase A from Aspergillus aculeatus: a right-handed parallel β helix”. Structure 5: 533-544. PMID 9115442.
↑ Kofod, L.V., Kauppinen, S., Christgau, S., Andersen, L.N., Heldt-Hansen, H.P., Dorreich, K. and Dalboge, H. (1994). „Cloning and characterization of two structurally and functionally divergent rhamnogalacturonases from Aspergillus aculeatus”. J. Biol. Chem. 269: 29182-29189. PMID 7961884.
↑ Azadi, P., O'Neill, M.A., Bergmann, C., Darvill, A.G. and Albersheim, P. (1995). „The backbone of the pectic polysaccharide rhamnogalacturonan I is cleaved by an endohydrolase and an endolyase”. Glycobiology 5: 783-789. PMID 8720076.
↑ Petersen, T.N., Christgau, S., Kofod, L.V., Kauppinen, S., Johnson, A.H. and Larsen, S. (1997). „Crystallization and preliminary X-ray studies of rhamnogalacturonase A from Aspergillus aculeatus”. Acta Crystallogr. D Biol. Crystallogr. 53: 105-107. PMID 15299976.
↑ Pitson, S.M., Mutter, M., van den Broek, L.A., Voragen, A.G. and Beldman, G. (1998). „Stereochemical course of hydrolysis catalysed by α-L-rhamnosyl and α-D-galacturonosyl hydrolases from Aspergillus aculeatus”. Biochem. Biophys. Res. Commun. 242: 552-559. PMID 9464254.

Literatura

Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.

Vanjske veze

MeSH Rhamnogalacturonan+hydrolase

[1] Petersen, T.N., Kauppinen, S. and Larsen, S. (1997). „The crystal structure of rhamnogalacturonase A from Aspergillus aculeatus: a right-handed parallel β helix”. Structure 5: 533-544. PMID 9115442.

[2] Kofod, L.V., Kauppinen, S., Christgau, S., Andersen, L.N., Heldt-Hansen, H.P., Dorreich, K. and Dalboge, H. (1994). „Cloning and characterization of two structurally and functionally divergent rhamnogalacturonases from Aspergillus aculeatus”. J. Biol. Chem. 269: 29182-29189. PMID 7961884.

[3] Azadi, P., O'Neill, M.A., Bergmann, C., Darvill, A.G. and Albersheim, P. (1995). „The backbone of the pectic polysaccharide rhamnogalacturonan I is cleaved by an endohydrolase and an endolyase”. Glycobiology 5: 783-789. PMID 8720076.

[4] Petersen, T.N., Christgau, S., Kofod, L.V., Kauppinen, S., Johnson, A.H. and Larsen, S. (1997). „Crystallization and preliminary X-ray studies of rhamnogalacturonase A from Aspergillus aculeatus”. Acta Crystallogr. D Biol. Crystallogr. 53: 105-107. PMID 15299976.

[5] Pitson, S.M., Mutter, M., van den Broek, L.A., Voragen, A.G. and Beldman, G. (1998). „Stereochemical course of hydrolysis catalysed by α-L-rhamnosyl and α-D-galacturonosyl hydrolases from Aspergillus aculeatus”. Biochem. Biophys. Res. Commun. 242: 552-559. PMID 9464254.

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