Kofein sintaza

Kofein sintaza
Identifikatori
EC broj 2.1.1.160
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB RCSB PDB PDBe PDBj PDBsum

Kofein sintaza (EC 2.1.1.160, dimetilksantinska metiltransferaza, 3N-metiltransferaza, DXMT, CCS1, S-adenozil-L-metionin:3,7-dimetilksantin 1-N-metiltransferaza) je enzim sa sistematskim imenom S-adenozil-L-metionin:3,7-dimetilksantin N1-metiltransferaza.[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju

(1) S-adenozil-L-metionin + 3,7-dimetilksantin S-adenozil-L-homocistein + 1,3,7-trimetilksantin
(2) S-adenozil-L-metionin + 1,7-dimetilksantin S-adenozil-L-homocistein + 1,3,7-trimetilksantin
(3) S-adenozil-L-metionin + 7-metilksantin S-adenozil-L-homocistein + 3,7-dimetilksantin

Paraksantin je najbolji supstrat ovog enzima.

Reference

  1. ↑ Kato, M., Mizuno, K., Fujimura, T., Iwama, M., Irie, M., Crozier, A. and Ashihara, H. (1999). „Purification and characterization of caffeine synthase from tea leaves”. Plant Physiol. 120: 579-586. PMID 10364410. 
  2. ↑ Mizuno, K., Okuda, A., Kato, M., Yoneyama, N., Tanaka, H., Ashihara, H. and Fujimura, T. (2003). „Isolation of a new dual-functional caffeine synthase gene encoding an enzyme for the conversion of 7-methylxanthine to caffeine from coffee (Coffea arabica L.)”. FEBS Lett. 534: 75-81. PMID 12527364. 
  3. ↑ Uefuji, H., Ogita, S., Yamaguchi, Y., Koizumi, N. and Sano, H. (2003). „Molecular cloning and functional characterization of three distinct N-methyltransferases involved in the caffeine biosynthetic pathway in coffee plants”. Plant Physiol. 132: 372-380. PMID 12746542. 
  4. ↑ Kato, M., Mizuno, K., Crozier, A., Fujimura, T. and Ashihara, H. (2000). „Caffeine synthase gene from tea leaves”. Nature 406: 956-957. PMID 10984041. 

Literatura

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