D-Man-alfa-(1-3)-D-Glc-beta-(1-4)-D-Glc-alfa-1-difosfoundekaprenol 2-beta-glukuroniltransferaza

D-Man-alfa-(1->3)-D-Glc-beta-(1->4)-D-Glc-alfa-1-difosfoundekaprenol 2-beta-glukuroniltransferaza
Identifikatori
EC broj 2.4.1.264
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB RCSB PDB PDBe PDBj PDBsum

D-Man-alfa-(1->3)-D-Glc-beta-(1->4)-D-Glc-alfa-1-difosfoundekaprenol 2-beta-glukuroniltransferaza (EC 2.4.1.264, GumK) je enzim sa sistematskim imenom UDP-glukuronat:D-Man-alfa-(1->3)-D-Glc-beta-(1->4)-D-Glc-alfa-1-difosfo-ditrans,oktacis-undekaprenol beta-1,2-glukuroniltransferaza.[1][2][3][4][5][6][7] Ovaj enzim katalizuje sledeću hemijsku reakciju

UDP-glukuronat + D-Man-alfa-(1->3)-D-Glc-beta-(1->4)-D-Glc-alfa-1-difosfo-ditrans,oktacis-undekaprenol UDP + D-GlcA-beta-(1->2)-D-Man-alfa-(1->3)-D-Glc-beta-(1->4)-D-Glc-alfa-1-difosfo-ditrans,oktacis-undekaprenol

Ovaj enzim učestvuje u biosintezi eksopolisaharida ksantana (kod bakterije Xanthomonas campestris) i acetana (kod bakterije Glukonacetobacter xylinus).

Reference

  1. ↑ Katzen, F., Ferreiro, D.U., Oddo, C.G., Ielmini, M.V., Becker, A., Puhler, A. and Ielpi, L. (1998). „Xanthomonas campestris' pv. campestris gum mutants: effects on xanthan biosynthesis and plant virulence”. J. Bacteriol. 180: 1607-1617. PMID 9537354. 
  2. ↑ Ielpi, L., Couso, R.O. and Dankert, M.A. (1993). „Sequential assembly and polymerization of the polyprenol-linked pentasaccharide repeating unit of the xanthan polysaccharide in Xanthomonas campestris”. J. Bacteriol. 175: 2490-2500. PMID 7683019. 
  3. ↑ Kim, S.Y., Kim, J.G., Lee, B.M. and Cho, J.Y. (2009). „Mutational analysis of the gum gene cluster required for xanthan biosynthesis in Xanthomonas oryzae' pv oryzae”. Biotechnol. Lett. 31: 265-270. PMID 18854951. 
  4. ↑ Barreras, M., Bianchet, M.A. and Ielpi, L. (2006). „Crystallization and preliminary crystallographic characterization of GumK, a membrane-associated glucuronosyltransferase from Xanthomonas campestris required for xanthan polysaccharide synthesis”. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 62: 880-883. PMID 16946469. 
  5. ↑ Barreras, M., Salinas, S.R., Abdian, P.L., Kampel, M.A. and Ielpi, L. (2008). „Structure and mechanism of GumK, a membrane-associated glucuronosyltransferase”. J. Biol. Chem. 283: 25027-25035. PMID 18596046. 
  6. ↑ Vojnov, A.A., Bassi, D.E., Daniels, M.J. and Dankert, M.A. (2002). „Biosynthesis of a substituted cellulose from a mutant strain of Xanthomonas campestris”. Carbohydr. Res. 337: 315-326. PMID 11841812. 
  7. ↑ Barreras, M., Abdian, P.L. and Ielpi, L. (2004). „Functional characterization of GumK, a membrane-associated β-glucuronosyltransferase from Xanthomonas campestris required for xanthan polysaccharide synthesis”. Glycobiology 14: 233-241. PMID 14736729. 

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