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5-epiaristolohen 1,3-dihidroksilaza
Identifikatori
EC broj	1.14.13.119
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB	RCSB PDB PDBe PDBj PDBsum
Pretraga
PMC	articles
PubMed	articles
NCBI Protein	search

5-epiaristolohen 1,3-dihidroksilaza (EC 1.14.13.119, 5-epi-aristolohenska 1,3-dihidroksilaza, EAH) je enzim sa sistematskim imenom 5-epiaristolohen,NADPH:kiseonik oksidoreduktaza (1- i 3-hidroksilacija).^[1]^[2] Ovaj enzim katalizuje sledeću hemijsku reakciju

5-epiaristolohen + 2 NADPH + 2 H⁺ + 2 O₂

\rightleftharpoons

kapsidiol + 2 NADP⁺ + 2H₂O

Ovaj enzim je hem-tiolatni protein (P-450). Kinetička istraživanja sugerišu da se uglavnom 1beta-hidroksiepiaristolohen prvo formira, i da tome sledi hidroksilacija na C-3.

Reference

↑ Ralston, L., Kwon, S.T., Schoenbeck, M., Ralston, J., Schenk, D.J., Coates, R.M. and Chappell, J. (2001). „Cloning, heterologous expression, and functional characterization of 5-epi-aristolochene-1,3-dihydroxylase from tobacco (Nicotiana tabacum)”. Arch. Biochem. Biophys. 393: 222-235. PMID 11556809.
↑ Takahashi, S., Zhao, Y., O'Maille, P.E., Greenhagen, B.T., Noel, J.P., Coates, R.M. and Chappell, J. (2005). „Kinetic and molecular analysis of 5-epiaristolochene 1,3-dihydroxylase, a cytochrome P₄₅₀ enzyme catalyzing successive hydroxylations of sesquiterpenes”. J. Biol. Chem. 280: 3686-3696. PMID 15522862.

Literatura

Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.

Vanjske veze

MeSH 5-epiaristolochene+1,3-dihydroxylase

[1] Ralston, L., Kwon, S.T., Schoenbeck, M., Ralston, J., Schenk, D.J., Coates, R.M. and Chappell, J. (2001). „Cloning, heterologous expression, and functional characterization of 5-epi-aristolochene-1,3-dihydroxylase from tobacco (Nicotiana tabacum)”. Arch. Biochem. Biophys. 393: 222-235. PMID 11556809.

[2] Takahashi, S., Zhao, Y., O'Maille, P.E., Greenhagen, B.T., Noel, J.P., Coates, R.M. and Chappell, J. (2005). „Kinetic and molecular analysis of 5-epiaristolochene 1,3-dihydroxylase, a cytochrome P₄₅₀ enzyme catalyzing successive hydroxylations of sesquiterpenes”. J. Biol. Chem. 280: 3686-3696. PMID 15522862.

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