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2,5-didehidroglukonat reduktaza
Identifikatori
EC broj	1.1.1.274
CAS broj	95725-95-4
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB	RCSB PDB PDBe PDBj PDBsum
Pretraga
PMC	articles
PubMed	articles
NCBI Protein	search

2,5-didehidroglukonat reduktaza (EC 1.1.1.274, 2,5-diketo-D-glukonat reduktaza, YqhE reduktaza) je enzim sa sistematskim imenom 2-dehidro-D-glukonat:NADP⁺ 2-oksidoreduktaza.^[1]^[2]^[3] Ovaj enzim katalizuje sledeću hemijsku reakciju

2-dehidro-D-glukonat + NADP⁺

\rightleftharpoons

2,5-didehidro-D-glukonat + NADPH + H⁺

Ovaj enzim je ključan u mikrobnoj produkciji askorbata. On može da stereoselektivno redukuje etil 2-metilacetoacetat do etil (2R)-metil-(3S)-hidroksibutanoata, a takođe može da deluje i na neke druge beta-keto estre. Identifikacija bakterijske beta-keto estarske reduktaze za 2,5-didehidroglukonat reduktazu povezuje dve prethodno zasebne oblasti biokatalize, i.e., asimetričnu karbonilnu redukciju i produkciju mikrobnog vitamina C.

Reference

↑ Yum, D.Y., Lee, B.Y. and Pan, J.G. (1999). „Identification of the yqhE and yafB genes encoding two 2,5-diketo-D-gluconate reductases in Escherichia coli”. Appl. Environ. Microbiol. 65: 3341-3346. PMID 10427017.
↑ Yum, D.Y., Lee, B.Y., Hahm, D.H. and Pan, J.G. (1998). „The yiaE gene, located at 80.1 minutes on the Escherichia coli chromosome, encodes a 2-ketoaldonate reductase”. J. Bacteriol. 180: 5984-5988. PMID 9811658.
↑ Habrych, M., Rodriguez, S. and Stewart, J.D. (2002). „Purification and identification of an Escherichia coli β-keto ester reductase as 2,5-diketo-D-gluconate reductase YqhE”. Biotechnol. Prog. 18: 257-261. PMID 11934293.

Literatura

Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.

Vanjske veze

MeSH 2,5-didehydrogluconate+reductase

[1] Yum, D.Y., Lee, B.Y. and Pan, J.G. (1999). „Identification of the yqhE and yafB genes encoding two 2,5-diketo-D-gluconate reductases in Escherichia coli”. Appl. Environ. Microbiol. 65: 3341-3346. PMID 10427017.

[2] Yum, D.Y., Lee, B.Y., Hahm, D.H. and Pan, J.G. (1998). „The yiaE gene, located at 80.1 minutes on the Escherichia coli chromosome, encodes a 2-ketoaldonate reductase”. J. Bacteriol. 180: 5984-5988. PMID 9811658.

[3] Habrych, M., Rodriguez, S. and Stewart, J.D. (2002). „Purification and identification of an Escherichia coli β-keto ester reductase as 2,5-diketo-D-gluconate reductase YqhE”. Biotechnol. Prog. 18: 257-261. PMID 11934293.

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