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TBST

"TTBS" redirects here. For the Ticket Tax Box Service, see International Air Transport Association.

In molecular biology, TBST (or TTBS) is a mixture of tris-buffered saline (TBS) (a buffer solution) and Polysorbate 20 (a polysorbate-type nonionic surfactant). Polysorbate 20 is also known as Tween 20, a commercial brand name. It is a common detergent used in many buffers for washing nitrocellulose membrane in western blotting and microtiter plate wells in ELISA assays. Tris is a buffer that maintains a pH of 7–9.2.

Contents of TBS-Tween

The following is a sample recipe for TBST:[1]

Adjust pH with HCl to pH 7.4–7.6

The simplest way to prepare a TBS-Tween solution is to use TBS-T tablets. They are formulated to give a ready to use TBST solution upon dissolution in 500 ml of deionized water.[2]

References

  1. ^ "Sigma aldrich TBST recipe". sigmaaldrich.com. Retrieved 2025-11-26.
  2. ^ "Medicago TBS Tween productsheet" (PDF). www.medicago.se. Archived (PDF) from the original on 2024-07-14. Retrieved 2025-11-26.

https://www.gbiosciences.com/Buffers-Reagents-Chemicals/Electrophoresis-Related-Buffers-Chemicals/TBST-10X


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