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The Women's 100 Freestyle swimming event at the 25th SEA Games was held on December 10, 2009.

Source: ^[1]

Place	Lane	Swimmer	Nation	Time	Notes
1	4	Quah Ting Wen	Singapore	56.03	GR
2	5	Natthanan Junkrajang	Thailand	56.33
3	6	Amanda Lim	Singapore	56.60
4	7	Jasmine Paler Alkhaldi	Philippines	57.43
5	3	Jiratida Phinyosophon	Thailand	57.46
6	2	Chui Lai Kwan	Malaysia	57.59
7	1	Leung Chii Lin	Malaysia	58.21
8	8	Enny Susilowati Margono	Indonesia	59.64

Rank	Heat/Lane	Swimmer	Nation	Time	Notes
1	H2 L4	Quah Ting Wen	Singapore	56.95	Q
2	H2 L5	Natthanan Junkrajang	Thailand	57.28	Q
3	H2 L3	Jiratida Phinyosophon	Thailand	57.58	Q
4	H1 L4	Amanda Lim	Singapore	57.96	Q
5	H2 L6	Chui Lai Kwan	Malaysia	58.25	Q
5	H1 L6	Jasmine Paler Alkhaldi	Philippines	58.25	Q
7	H1 L5	Leung Chii Lin	Malaysia	58.75	Q
8	H1 L3	Enny Susilowati Margono	Indonesia	59.50	Q
9	H1 L2	Heidi Gem Chua Ong	Philippines	59.79
10	H2 L2	Tran Tam Nguyen	Vietnam	1:00.02
11	H2 L1	Nguyen Thi Ngoc Yen	Vietnam	1:00.51
12	H2 L7	Ressa Kania Dewi	Indonesia	1:01.39
13	H1 L7	Hemthon Vitiny	Cambodia	1:08.71
14	H1 L1	Thepaksone Chindavong	Laos	1:14.64
15	H1 L8	Napha Xinsaivolavong	Laos	1:22.18