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Spirit blue
Names
	IUPAC name 4-[(4-anilinophenyl)-(4-phenyliminocyclohexa-2,5-dien-1-ylidene)methyl]-N-phenylaniline hydrochloride
	Other names Opal blue SS, HSDB 466, Solvent Blue 23 monohydrochloride
Identifiers
CAS Number	2152-64-9 ;
ChEBI	CHEBI:86483 ;
ChemSpider	15674 ;
ECHA InfoCard	100.016.766
PubChem CID	16534;
UNII	690DO205AX ;
CompTox Dashboard (EPA)	DTXSID3027457 ;
Properties
Chemical formula	C37H30ClN3
Molar mass	552.12 g·mol−1
	Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). Infobox references

Spirit blue or Opal blue SS is a dye with formula C₃₇H₃₀ClN₃.^[1] It is used as an indicator in agar for detection of lipase activity in bacteria.^[2]

References

^ "Opal Blue SS". National Center for Biotechnology Information. Archived from the original on 21 December 2016. Retrieved 21 December 2016.
^ Starr, Mortimer P. (April 4, 1941). "Spirit Blue Agar: A Medium for the Detection of Lipolytic Microorganisms". Science. 93 (2414): 333–334. Bibcode:1941Sci....93..333S. doi:10.1126/science.93.2414.333. PMID 17748334 – via CrossRef.

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[pubchem-1] "Opal Blue SS". National Center for Biotechnology Information. Archived from the original on 21 December 2016. Retrieved 21 December 2016.

[2] Starr, Mortimer P. (April 4, 1941). "Spirit Blue Agar: A Medium for the Detection of Lipolytic Microorganisms". Science. 93 (2414): 333–334. Bibcode:1941Sci....93..333S. doi:10.1126/science.93.2414.333. PMID 17748334 – via CrossRef.

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