Sister James can refer to:

• "Sister James", a 1973 song by Nino Tempo and 5th Ave. Sax
• Sister Marita James, a character in the 2004 play Doubt: A Parable and the 2008 movie Doubt
• James Charles, an American internet personality and makeup artist who served as the first male CoverGirl spokesperson

This disambiguation page lists articles associated with the title Sister James.
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