Regulatory region of repBA gene

Pseudoknot of the regulatory region of the repBA gene
Secondary structure of the pseudoknot of the regulatory region of the repBA gene
Identifiers
RfamRF01089
Other data
RNA typeCis-reg
Domain(s)Eukaryotes Prokaryotes
PDB structuresPDBe

In plasmids, the regulatory region of repBA gene forms a pseudoknot. The repA gene, which encodes a protein likely to function as an initiator for replication, and the repB gene are translationally coupled. The leader sequence of the repA mRNA contains two complementary sequences of 8 bases. Base-pairing between these two sequences forms a pseudoknot which is essential for translation. The first of these complementary sequences is found within a stem-loop, which forms a target for RNAI. Binding of RNAI to this stem-loop inhibits pseudoknot formation and translation of RepA.[1][2]

References

  1. ^ Athanasopoulos V, Praszkier J, Pittard AJ (August 1995). "The replication of an IncL/M plasmid is subject to antisense control". J. Bacteriol. 177 (16): 4730–4741. PMC 177239. PMID 7543895.
  2. ^ Athanasopoulos V, Praszkier J, Pittard AJ (March 1999). "Analysis of elements involved in pseudoknot-dependent expression and regulation of the repA gene of an IncL/M plasmid". J. Bacteriol. 181 (6): 1811–1819. PMC 93579. PMID 10074073.