Polyketide synthases (PKSs) of fungi are Type I PKSs. Monocerin has been confirmed by biosynthesis studies to have heptaketide origins. Monocerin PKS produces an intermediate with initially a high degree of reductive modification but ends with a classical β-polyketide moiety. Dihydroisocoumarin is the first PKS-free intermediate which would be formed from the reduced heptaketide whose assembly pathway is shown in figure 1.[2] Ketosynthase, ketoreductase, dehydrates, enol reductases and cyclisases are shown as domains of the Monocerin PKS and methyl transferase is considered to be a tailoring enzyme.[3]
Formation of an enolate ion on the carbon three carbons away from sulfur allows aldol addition onto the carbonyl six carbons distant along the chain. This produces the secondary alcohol. Dehydration proceeds to give the alkene. Enolization then occurs to reach the stability of the aromatic ring.[4]
The modified chain is transferred to the TE domain. This will allow lactonization and release from the enzyme.[4]: 72
Hydroxylation occurs at ortho-position to two substituents. O-methylation occurs.[1]
Monocerin produced by Exserohilum turcicum causes Northern Corn Leaf blight disease in maize. The maize will develop brown lesions on its leaves and will have decreased viability in its root cap cells.[5] Monocerin has also been shown to be an effective insecticide against wooly aphids.[6] Monocerin is also an effective herbicide against Johnson grass by inhibiting seedling growth. It has a lesser effect against cucumber.[7]
^Cuq, F., Brown, S.C., Petitprez, M., and Alibert, G. (1995). "Effects of monocerin on cell cycle progression in maize root meristems synchronized with aphidicolin". Plant Cell Reports. 15 (1–2): 138–142. doi:10.1007/bf01690271. PMID24185672. S2CID22721253.{{cite journal}}: CS1 maint: multiple names: authors list (link)
^Grove, J.F. & Pople M. (1979). "Metabolic Products of Fusarium larvarum Fuckel. The Fusarentins and the Absolute configuration of Monocerin". Journal of the Chemical Society, Perkin Transactions 1. 1: 2048–2051. doi:10.1039/p19790002048.
^Roberson, D.J. & Strobel, G.M. (1982). "Monocerin, a Phytotoxin from Exserohilum turcicum". Agricultural and Biological Chemistry. 46: 2681–2683.