In molecular biologymir-663 microRNA is a short RNA molecule. MicroRNAs function to regulate the expression levels of other genes by several mechanisms.
Gastric cancer suppression
miR-663 has been identified as a possible suppressor of tumour growth, with its levels downregulated in human gastric cancer cell lines. Its introduction into the two human gastric cancer cell lines BGC823 and SNU5 induces morphology changes and suppresses cell proliferation.[1] Transfection with miR-663 also sees a resultant upregulation of cyclin B.
miR-155 upregulation and other molecular targets
Resveratrol, a natural phenol and antioxidant, upregulates miR-663 in human THP-1 monocytic cells, human blood monocytes and MCF7 brest cancer cells.[2] Endogenous activator protein-1 (AP-1) activity is decreased by miR-663 and there is additional impaired lipopolysaccharide upregulation. miR-663 directly targets JunD and JunB transcripts, and alters AP-1 upregulation through this. It is further involved in the impaired lipopolysaccharide upregulation of miR-155 by resveratrol.[3] miR-663 also directly targets EEF1A2, translation elongation factor and well-known protooncogene.[2][4]
^Anand N, Murthy S, Amann G, Wernick M, Porter LA, Cukier IH, Collins C, Gray JW, Diebold J, Demetrick DJ, Lee JM (July 2002). "Protein elongation factor EEF1A2 is a putative oncogene in ovarian cancer". Nature Genetics. 31 (3): 301–5. doi:10.1038/ng904. PMID12053177. S2CID37997742.
Further reading
Liang P, Lv C, Jiang B, Long X, Zhang P, Zhang M, Xie T, Huang X (June 2012). "MicroRNA profiling in denatured dermis of deep burn patients". Burns. 38 (4): 534–40. doi:10.1016/j.burns.2011.10.014. PMID22360957.
Lu J, Kwan BC, Lai FM, Tam LS, Li EK, Chow KM, Wang G, Li PK, Szeto CC (May 2012). "Glomerular and tubulointerstitial miR-638, miR-198 and miR-146a expression in lupus nephritis". Nephrology. 17 (4): 346–51. doi:10.1111/j.1440-1797.2012.01573.x. PMID22295894. S2CID24163790.
Yang Y, Wang LL, Li YH, Gao XN, Liu Y, Yu L (February 2012). "Effect of CpG island methylation on microRNA expression in the k-562 cell line". Biochemical Genetics. 50 (1–2): 122–34. doi:10.1007/s10528-011-9478-9. PMID22089542. S2CID16736093.
Yang Y, Wang LL, Li YH, Gao XN, Yu L (April 2011). "[Expression level of miRNA-663 in different leukemic cell lines and its biological function]". Zhongguo Shi Yan Xue Ye Xue Za Zhi. 19 (2): 279–83. PMID21518471.
Liu ZY, Zhang GL, Wang MM, Xiong YN, Cui HQ (2011). "MicroRNA-663 targets TGFB1 and regulates lung cancer proliferation". Asian Pacific Journal of Cancer Prevention. 12 (11): 2819–23. PMID22393947.
Maes OC, Sarojini H, Wang E (October 2009). "Stepwise up-regulation of microRNA expression levels from replicating to reversible and irreversible growth arrest states in WI-38 human fibroblasts". Journal of Cellular Physiology. 221 (1): 109–19. doi:10.1002/jcp.21834. PMID19475566. S2CID21566082.
Pizzimenti S, Ferracin M, Sabbioni S, Toaldo C, Pettazzoni P, Dianzani MU, Negrini M, Barrera G (January 2009). "MicroRNA expression changes during human leukemic HL-60 cell differentiation induced by 4-hydroxynonenal, a product of lipid peroxidation". Free Radical Biology & Medicine. 46 (2): 282–8. doi:10.1016/j.freeradbiomed.2008.10.035. PMID19022373.
Lehmann U, Hasemeier B, Römermann D, Müller M, Länger F, Kreipe H (2007). "[Epigenetic inactivation of microRNA genes in mammary carcinoma]". Verhandlungen der Deutschen Gesellschaft für Pathologie. 91: 214–20. PMID18314617.
Lehmann U, Hasemeier B, Christgen M, Müller M, Römermann D, Länger F, Kreipe H (January 2008). "Epigenetic inactivation of microRNA gene hsa-mir-9-1 in human breast cancer". The Journal of Pathology. 214 (1): 17–24. doi:10.1002/path.2251. PMID17948228. S2CID22024957.