In enzymology, a manganese-transporting ATPase (EC 3.6.3.35) is an enzyme that catalyzes the chemical reaction

ATP + H₂O + Mn₂+out ${\displaystyle \rightleftharpoons }$ ADP + phosphate + Mn₂+in

The 3 substrates of this enzyme are ATP, H₂O, and Mn²⁺, whereas its 3 products are ADP, phosphate, and Mn²⁺.

This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides to catalyse transmembrane movement of substances. The systematic name of this enzyme class is called ATP phosphohydrolase (manganese-importing). This enzyme is also known as ABC-type manganese permease complex.

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