The painting was made for the church of the now deconsecrated Franciscan Monastery of Santi Ludovico e Alessio, located on Via del Pratello, in Bologna. It depicts the Virgin above seated on a cloud in glory with her infant Jesus, while below gather, from left to right, Saints Clare of Assisi, Louis IX of France, Francis of Assisi, a young John the Baptist, Alexius of Rome, and Catherine of Alexandria.