Lavendamycin was first discovered in 1981 by Doyle et al., who isolated it from Streptomyces lavendulae.[3] As the compound failed to crystallize, a direct characterization of the molecular structure with X-ray crystallography was not possible. Careful analysis using NMR, IR, and UV-VIS spectroscopy and mass spectrometry allowed the assignment of the pentacyclic structure consisting of a β-carboline unit and a quinolinequinone unit.[citation needed]
Total syntheses
The attractive biological properties and complex structure of lavendamycin have made it the target of a large number of total syntheses.[4] Within a few years after the structural elucidation by Doyle et al., the research groups of Kende,[5] Hibino,[6] Rao,[7] and Boger[8] had already developed total syntheses for the compound independently of one another. The discovery that analogs of lavendamycin are potent inhibitors of HIV reverse transcriptase led to further attempts in the 90s to develop efficient routes to lavendamycin.[9][10][11][12] However, large numbers of steps, low overall yields (0.5–2%) or poorly available starting materials make these syntheses unattractive for further systematic development of lavendamycin and its analogs. Notably, total syntheses by Behforouz[13] and Nissen[14] offer flexible construction of the lavendamycin scaffold at high yields.
^ abHassani, M.; Cai, W.; Koelsch, K. H.; Holley, D. C.; Rose, A. S.; Olang, F.; Lineswala, J. P.; Holloway, W. G.; Gerdes, J. M.; Behforouz, M.; Beall, H. D. (2008). "Lavendamycin antitumor agents: Structure-based design, synthesis, and NAD(P)H:quinone oxidoreductase 1 (NQO1) model validation with molecular docking and biological studies". Journal of Medicinal Chemistry. 51 (11): 3104–15. doi:10.1021/jm701066a. PMID18457384.