Karnovsky fixative
Karnovsky fixative, developed by the pathologist Morris J. Karnovsky (1926–2018), is a fixative for electron microscopy.[1]
Solution
The stock solution for Karnovsky fixative is as follows:[1]
- 2.0 g paraformaldehyde
- 25 ml distilled water
- 1M sodium hydroxide 2 to 4 drops
- 50% glutaraldehyde 5.0 ml
- 0.2M cacodylate buffer, pH 7.4, 20.0 ml
Mix the paraformaldehyde with 25 ml of distilled water in a 125 ml Erlenmeyer flask. Heat to 60 °C on a stir plate. When moisture forms on the sides of flask, add sodium hydroxide and stir until the solution clears. Cool solution under the faucet. Filter, add glutaraldehyde and 0.2M buffer, pH range 7.2 to 7.4.
Modified solutions
Karnovsky's fixative solution is often modified for specialized applications.[2][3] For example, 2% paraformaldehyde, 2.5% glutaraldehyde in 0.1 Molar sodium phosphate buffer (pH 7.4) has been used to study the ultrastructure of renal pelvis fragments.[3]
References
- ^ a b Karnovsky fixative
- ^ Shah FA, Johansson BR, Thomsen P, Palmquist A (2015). "Ultrastructural evaluation of shrinkage artefacts induced by fixatives and embedding resins on osteocyte processes and pericellular space dimensions". Journal of Biomedical Materials Research. 103 (4): 1565–1576. doi:10.1002/jbm.a.35287. PMID 25046613.
- ^ a b Favorito LA, Costa WS, Lobo M, Gallo CM, Sampaio FJ (2020). "Morphology of the fetal renal pelvis during the second trimester: Comparing genders". Journal of Pediatric Surgery. doi:10.1016/j.jpedsurg.2019.12.029. PMID 32037216.
External links
Content Disclaimer
Informasi ini disarikan dari Wikipedia dan disajikan kembali untuk tujuan edukasi. Konten tersedia di bawah lisensi CC BY-SA 3.0. Kami tidak bertanggung jawab atas ketidakakuratan data yang bersumber dari kontribusi publik tersebut.
- The information displayed on this website is sourced in part or in whole from Wikipedia and has been adapted for the purpose of restating it. We strive to provide accurate and relevant information, however:
- There is no guarantee of absolute accuracy. Wikipedia is an open, collaborative project that can be edited by anyone, so information is subject to change.
- It is not intended to constitute professional advice. The content displayed is for informational and educational purposes only. For important decisions (e.g., medical, legal, or financial), please consult a professional.
- Content copyright. Wikipedia is licensed under the Creative Commons Attribution-ShareAlike License (CC BY-SA). This means that content may be reused with appropriate attribution and shared under a similar license.
- Responsible use. Any risk arising from the use of information from this website is entirely the responsibility of the user.